前列腺素E的放射免疫测定及前列腺素E1特异性测量方法

Radioimmunoassay of PGE and an approach to the specific measurement of PGE1.

作者信息

Van Orden D E, Farley D B, Clancey C J

出版信息

Prostaglandins. 1977 Mar;13(3):437-53. doi: 10.1016/0090-6980(77)90024-7.

Abstract

A specific radioimmunoassay for prostaglandins of the E series is presented. Several refinements of the conventional procedures used for unknown sample preparation prior to assay are described. Validation of the method through recovery of labeled and unlabeled prostaglandin E from plasma indicates: 74% extraction efficiency, 80% chromatographic separation efficiency, and a 67% overall recovery figure for the complete assay. The development of an "absorbed system" approach for measuring one E prostaglandin (PGE1) without interference from the other E prostaglandin (PGE2) is also described. A preliminary study of rat tissue and male plasma samples measured by these assay systems is included. Alternate approaches to the development of a specific PGE1 assay, although less feasible in these authors' hands, are discussed.

摘要

本文介绍了一种针对E系列前列腺素的特异性放射免疫测定法。文中描述了在测定未知样品之前，对常规样品制备程序进行的若干改进。通过从血浆中回收标记和未标记的前列腺素E来验证该方法，结果表明：提取效率为74%，色谱分离效率为80%，整个测定的总回收率为67%。还描述了一种“吸收系统”方法的开发，该方法可用于测量一种E前列腺素（PGE1），而不受另一种E前列腺素（PGE2）的干扰。文中还包括了用这些测定系统对大鼠组织和雄性血浆样品进行的初步研究。讨论了开发特异性PGE1测定法的其他方法，尽管在这些作者手中不太可行。