绵羊寄生线虫捻转血矛线虫发育过程中的蜕皮甾体类化合物
Ecdysteroids during development in the ovine parasitic nematode, Haemonchus contortus.
作者信息
Fleming M W
机构信息
Helminthic Diseases Laboratory, Livestock and Poultry Sciences Institute, U.S. Department of Agriculture, Beltsville, MD 20705.
出版信息
Comp Biochem Physiol B. 1993 Apr;104(4):653-5. doi: 10.1016/0305-0491(93)90192-8.
PMID:8472534
Abstract
- Samples of embryonating eggs, infective larvae, fourth-stage larvae, young adults, and male and female mature adults of Haemonchus contortus were collected for steroids analysis; lipids were extracted, partially purified, fractionated with HPLC, and analyzed for ecdysteroids by radioimmunoassay. 2. Free ecdysone and 20-hydroxyecdysone were detected in all samples; maximal concentrations occurred in the fourth-stage larvae and young adults. 3. Conjugated ecdysone and 20-hydroxyecdysone were detected in all samples but the infected larvae; maximal concentrations occurred in the fourth-stage larvae and young adults. 4. Patterns of ecdysteroid occurrence support regulatory roles in embryogenesis, cuticular deposition, and gonadogenesis. 5. Relative concentrations of ecdysteroids are comparable to those previously reported in eggs and adults of Ascaris suum.
摘要
- 收集捻转血矛线虫的胚胎卵、感染性幼虫、第四期幼虫、年轻成虫以及雌雄成熟成虫样本用于类固醇分析；提取脂质，进行部分纯化，用高效液相色谱法分离，并通过放射免疫分析法分析蜕皮甾体。2. 在所有样本中均检测到游离蜕皮激素和20-羟基蜕皮激素；最高浓度出现在第四期幼虫和年轻成虫中。3. 在除感染幼虫外的所有样本中均检测到结合蜕皮激素和20-羟基蜕皮激素；最高浓度出现在第四期幼虫和年轻成虫中。4. 蜕皮甾体的出现模式支持其在胚胎发生、表皮沉积和性腺发生中的调节作用。5. 蜕皮甾体的相对浓度与先前报道的猪蛔虫卵和成虫中的浓度相当。
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