黄胸散白蚁（科拉尔）分离工蚁中形态发生激素滴度的变化

Changes in morphogenetic hormone titers in isolated workers of the termite Reticulitermes flavipes (Kollar).

作者信息

Okot-Kotber B M, Prestwich G D, Strambi A, Strambi C

机构信息

Department of Chemistry, State University of New York, Stony Brook 11794-3400.

出版信息

Gen Comp Endocrinol. 1993 Jun;90(3):290-5. doi: 10.1006/gcen.1993.1084.

DOI:10.1006/gcen.1993.1084

PMID:8224756

Abstract

The daily titers of juvenile hormone and ecdysteroids were determined for workers of the Eastern subterranean termite Reticulitermes flavipes (Kollar) (Isoptera, Rhinotermitidae) following isolation of the workers from soldiers and reproductives. Experiments have demonstrated that isolation leads to biochemical and physiological changes that result in a presolider molt. Juvenile hormone (JH) and molting hormone (MH) titers were determined in hemolymph samples using radioimmunoassay. The identity of the ecdysteroids as predominantly 20-hydroxyecdysone (90%) and ecdysone (10%) was determined by HPLC using pooled extracts of hemolymph.

摘要

在将东方地下白蚁黄胸散白蚁（Reticulitermes flavipes (Kollar)，等翅目，鼻白蚁科）的工蚁与兵蚁和繁殖蚁分离后，测定了其保幼激素和蜕皮甾体的每日滴度。实验表明，分离会导致生化和生理变化，从而引发前兵蚁蜕皮。使用放射免疫分析法测定血淋巴样本中的保幼激素（JH）和蜕皮激素（MH）滴度。通过高效液相色谱法（HPLC），利用血淋巴混合提取物确定蜕皮甾体主要为20-羟基蜕皮酮（90%）和蜕皮酮（10%）。