Barer M R, Millership S E, Tabaqchali S
J Med Microbiol. 1986 Dec;22(4):303-9. doi: 10.1099/00222615-22-4-303.
Ninety-five strains of Aeromonas were divided into three species--A. sobria, A. hydrophila and A. caviae--on the basis of results in 13 biochemical tests. The minimum number of tests necessary to distinguish these species was determined. Culture filtrate of the strains were tested for cytotoxin and cytotonin, haemolysin and protease. One filtrate with high-titre cytotoxin, haemolysin and protease activities was subjected to gel filtration on Sephadex G75 and isoelectric focussing. Of the five cell lines tested, Vero cells were most sensitive to the cytotoxin; no reproducible cytotonic effects were observed. The haemolysin effect appeared to be equivalent to cytotoxin. At least two distinct protease activities were found that might be responsible for the cytotonic effects described. Cytotoxin production was species related; it was present in A. sobria and A. hydrophila but not in A. caviae.
根据13项生化试验的结果，95株气单胞菌被分为三个种——温和气单胞菌、嗜水气单胞菌和豚鼠气单胞菌。确定了区分这些种所需的最少试验次数。对这些菌株的培养滤液进行了细胞毒素和细胞紧张素、溶血素和蛋白酶检测。对一种具有高滴度细胞毒素、溶血素和蛋白酶活性的滤液进行了Sephadex G75凝胶过滤和等电聚焦。在所检测的5种细胞系中，Vero细胞对细胞毒素最敏感；未观察到可重复的细胞紧张效应。溶血素效应似乎与细胞毒素相当。发现至少有两种不同的蛋白酶活性可能是所描述的细胞紧张效应的原因。细胞毒素的产生与种有关；它存在于温和气单胞菌和嗜水气单胞菌中，但不存在于豚鼠气单胞菌中。
