一种与志贺毒素交叉反应的针对痢疾志贺氏菌13型的单克隆抗体。
A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin.
作者信息
Qadri F, Azim T, Hossain A, Islam D, Mondol G, Faruque S M, Albert M J
机构信息
International Centre for Diarrhoeal Disease Research, Bangladesh, (ICDDR,B), Dhaka.
出版信息
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):343-7. doi: 10.1111/j.1574-6968.1993.tb06055.x.
A monoclonal antibody (mAb ICT6) was produced against the newly described Shigella dysenteriae serotype type 13. The mAb was of IgM isotype and recognized purified Shiga toxin in ELISA and immunoblot. It also recognized periplasmic extract S. dysenteriae type 13 in immunoblot as did an affinity-purified polyclonal rabbit antiserum and a previously described monoclonal antibody to the B subunit of Shiga toxin. The mAb ICT6 did not neutralize the cytotoxic effects or S. dysenteriae type 13, Shiga toxin or periplasmic extracts of S. dysenteriae type 1 for HeLa cells.
制备了一种针对新描述的痢疾志贺氏菌血清型13的单克隆抗体（mAb ICT6）。该单克隆抗体为IgM同种型，在酶联免疫吸附测定（ELISA）和免疫印迹中可识别纯化的志贺毒素。在免疫印迹中，它还能识别痢疾志贺氏菌13型的周质提取物，亲和纯化的兔多克隆抗血清以及先前描述的针对志贺毒素B亚基的单克隆抗体也能识别。mAb ICT6不能中和痢疾志贺氏菌13型、志贺毒素或痢疾志贺氏菌1型周质提取物对HeLa细胞的细胞毒性作用。
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