Vitovski S
School of Biological Sciences, University of Wales, Bangor, Gwynedd, UK.
FEMS Microbiol Lett. 1993 Mar 15;108(1):1-5. doi: 10.1016/0378-1097(93)90477-j.
Nine different bacterial strains that utilise phenylacetic acid as the only carbon and energy source were isolated from samples of different geographical origin. The isolates were characterised taxonomically and physiologically. Evidence is presented that in all the isolates as well as in four previously isolated control strains with the ability to utilize phenylacetic acid, the enzyme phenylacetate-CoA ligase is specifically induced during growth on phenylacetic acid. The Michaelis constant (Km) in one Pseudomonas strain was sufficiently low (-1 mM) to suggest that the enzyme may have a role in phenylacetic acid metabolism.
从不同地理来源的样本中分离出九种不同的细菌菌株,它们仅以苯乙酸作为唯一的碳源和能源。对这些分离菌株进行了分类学和生理学特征分析。有证据表明,在所有这些分离菌株以及之前分离出的四种具有利用苯乙酸能力的对照菌株中,苯乙酸 - 辅酶A连接酶在以苯乙酸为碳源生长期间被特异性诱导。在一种假单胞菌菌株中,米氏常数(Km)足够低(-1 mM),这表明该酶可能在苯乙酸代谢中发挥作用。