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两个新的fli基因的克隆与测序,其产物对沙门氏菌鞭毛生物合成至关重要。

Cloning and sequencing of two new fli genes, the products of which are essential for Salmonella flagellar biosynthesis.

作者信息

Doll L, Frankel G

机构信息

Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Gene. 1993 Apr 15;126(1):119-21. doi: 10.1016/0378-1119(93)90599-x.

Abstract

We have identified two new open reading frames downstream from fliC, which appear to encode 19- and 20-kDa proteins that are essential for flagellar formation. The nucleotide and the predicted amino acid sequences of the genes are presented. Both genes, termed fliU and fliV, are preceded by the flagellar-specific -10 consensus promoter DNA sequence, suggesting their expression in the earlier stages of flagellar-component biosynthesis.

摘要

我们在fliC下游鉴定出两个新的开放阅读框,它们似乎编码对鞭毛形成至关重要的19 kDa和20 kDa蛋白质。文中呈现了这些基因的核苷酸序列和预测的氨基酸序列。这两个基因分别称为fliU和fliV,其前面均有鞭毛特异性的-10共有启动子DNA序列,这表明它们在鞭毛成分生物合成的早期阶段表达。

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