Labarrière N, Piau J P, Zennadi R, Blanchardie P, Denis M, Lustenberger P
Department of Medical Biochemistry, INSERM CJF-90.11, Nantes, France.
In Vitro Cell Dev Biol. 1993 Feb;29A(2):140-4. doi: 10.1007/BF02630945.
We examined the effects of all-trans retinoic acid (RA) on alpha(1-->2) fucosyltransferase activity and sensitivity to LAK-mediated cytotoxicity in two rat colon carcinoma cell lines differing by their glycosylation state and their tumorigenic potential. RA induced a decrease in alpha(1-->2) fucosyltransferase activity in the more tumorigenic variant PROb. Fucosyltransferase mRNA levels were not affected by RA treatment in PROb cells, suggesting a posttranscriptional control. This inhibition was accompanied by a decreased expression of fucosylated membrane glycoconjugates and by a significant increase in the sensitivity to LAK-mediated cytotoxicity. REGb cells, which exhibited a very low enzymatic activity and very few fucosylated glycoconjugates, were more sensitive to LAK-lysis than PROb cells and were not affected by RA treatment.
我们研究了全反式维甲酸（RA）对两种大鼠结肠癌细胞系中α（1→2）岩藻糖基转移酶活性以及对LAK介导的细胞毒性敏感性的影响，这两种细胞系在糖基化状态和致瘤潜能方面存在差异。RA导致致瘤性更强的变体PROb细胞中α（1→2）岩藻糖基转移酶活性降低。在PROb细胞中，岩藻糖基转移酶mRNA水平不受RA处理的影响，提示存在转录后调控。这种抑制伴随着岩藻糖化膜糖缀合物表达的降低以及对LAK介导的细胞毒性敏感性的显著增加。REGb细胞表现出非常低的酶活性和极少的岩藻糖化糖缀合物，其对LAK裂解的敏感性高于PROb细胞，且不受RA处理的影响。
