Hagiwara S, Umeda M, Tominaga Y, Takamatsu N, Yoshida N
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1995 Dec;62(4):495-505. doi: 10.5357/koubyou.62.495.
The present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of Periocline on these periodontopathic bacteria. Samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. After sampling, Periocline was applied topically to the selected pockets once a week for four weeks and reexamined. The detected rates of the periodontopathic bacteria were Capnocytophaga sputigena (37.1%), Prevotella intermedia (22.6%), Porphyromonas gingivalis (22.6%), Fusobacterium nucleatum (20.1%), Actinobacillus actinomycetemcomitans (9.7%) and Eikenella corrodens (4.8%). The distribution of the bacteria was compound because two or three bacterial species were found to coexist. In view of the MIC of minocycline-HCI for these bacteria, increase of most of the measured bacteria was suppressed by the concentration of drugs, including Periocline. However, clinical strains of P. i. were considered to have low susceptibility to minocycline-HCl. In view of the effect of topical application of drugs, no significant differences were found. From these results, it was suggested that Periocline contained effective concentration of minocycline-HCl.
本研究旨在检测六种牙周病原菌在牙周深袋中的分布情况，并再次确认派丽奥对这些牙周病原菌的作用。从21例年龄在43至75岁的牙周炎患者的62个牙周袋中收集样本，这些牙周袋深度超过4mm。采样后，每周一次对选定的牙周袋局部应用派丽奥，持续四周，然后再次检查。牙周病原菌的检出率分别为：牙龈二氧化碳嗜纤维菌（37.1%）、中间普氏菌（22.6%）、牙龈卟啉单胞菌（22.6%）、具核梭杆菌（20.1%）、伴放线放线杆菌（9.7%）和腐蚀埃肯菌（4.8%）。细菌分布呈复合型，因为发现有两三种细菌共存。鉴于盐酸米诺环素对这些细菌的最低抑菌浓度，包括派丽奥在内的药物浓度抑制了大多数被测细菌的增加。然而，中间普氏菌的临床菌株被认为对盐酸米诺环素敏感性较低。鉴于药物局部应用的效果，未发现显著差异。从这些结果表明，派丽奥含有有效浓度的盐酸米诺环素。
