Kudo C, Yamashita T, Araki A, Terashita M, Watanabe T, Atsumi M, Tamura M, Sendo F
Department of Parasitology, Yamagata University School of Medicine, Japan.
J Immunol. 1993 May 1;150(9):3728-38.
Recent studies on neutrophils have revealed that these cells produce various cytokines, and may be involved in regulation of the immune response. We examined whether neutrophils are involved in delayed type hypersensitivity (DTH) to SRBC in rats by selective depletion of in vivo neutrophils using a mAb designated RP-3. When the rats had been treated with RP-3 at the time of priming with SRBC, DTH to these cells was inhibited. Furthermore, RP-3 treatment was effective in inhibiting the effector phase of the DTH response to SRBC. When spleen cells from rats that had been treated with RP-3 at the time of immunization were used for local transfer of DTH, footpad swelling was significantly less than that induced by spleen cells from the RP-3-untreated immune rats.
近期对中性粒细胞的研究表明，这些细胞可产生多种细胞因子，并可能参与免疫反应的调节。我们使用一种名为RP - 3的单克隆抗体在体内选择性清除中性粒细胞，以此研究大鼠对SRBC的迟发型超敏反应（DTH）中中性粒细胞是否参与其中。在用SRBC进行初次免疫时用RP - 3处理大鼠，对这些细胞的DTH受到抑制。此外，RP - 3处理在抑制对SRBC的DTH反应的效应阶段方面是有效的。当将免疫时用RP - 3处理的大鼠的脾细胞用于DTH的局部转移时，足垫肿胀明显小于未用RP - 3处理的免疫大鼠的脾细胞所诱导的肿胀。
