Tamura M, Sekiya S, Terashita M, Sendo F
Department of Immunology and Parasitology, Yamagata University School of Medicine, Japan.
J Immunol. 1994 Aug 1;153(3):1301-8.
Because recent work has shown that neutrophils produce various cytokines and are activated by these agents, this study was undertaken to examine neutrophil participation in immune networks. In our previous work, we demonstrated that delayed type hypersensitivity to SRBC and accompanying mononuclear leukocyte recruitment are inhibited in vivo by selective depletion of neutrophils using a mAb designated RP-3. To elucidate the function of these cells in Ab production, we assessed the splenic plaque-forming cell response to SRBC in a rat model through selective depletion of circulating neutrophils by RP-3. This depletion which occurred at the time of immunization resulted in an increase in the number of anti-SRBC Ab producing cells, as determined by the direct and indirect plaque-forming cell response. This phenomenon was observed only when the Ag was administered i.p. and not with i.v. immunization. These results suggest that neutrophils suppress Ab production in certain situations.
由于最近的研究表明中性粒细胞可产生多种细胞因子并被这些因子激活，因此开展了本研究以检测中性粒细胞在免疫网络中的参与情况。在我们之前的工作中，我们证明，使用一种名为RP-3的单克隆抗体选择性耗竭中性粒细胞，可在体内抑制对SRBC的迟发型超敏反应以及伴随的单核白细胞募集。为了阐明这些细胞在抗体产生中的功能，我们通过RP-3选择性耗竭循环中的中性粒细胞，在大鼠模型中评估了脾脏对SRBC的空斑形成细胞反应。免疫时发生的这种耗竭导致抗SRBC抗体产生细胞数量增加，这是通过直接和间接空斑形成细胞反应确定的。仅当抗原经腹腔注射而非静脉注射免疫时才观察到这种现象。这些结果表明，在某些情况下中性粒细胞会抑制抗体产生。
