Bellas R E, Hopkins N, Li Y
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
J Virol. 1993 May;67(5):2908-13. doi: 10.1128/JVI.67.5.2908-2913.1993.
We demonstrate here that the nuclear factor-kappa B (NF-kappa B) binding site in the simian immunodeficiency virus (SIVmac) long terminal repeat is essential for efficient virus replication in primary alveolar macrophages but dispensable for efficient replication in primary T cells. Mutation of the NF-kappa B site does not seriously impair replication of a T-cell-tropic SIVmac239 or a macrophagetropic SIVmacEm* in peripheral blood lymphocytes or established CD4+ cell lines; however, mutation of the NF-kappa B site prevents efficient SIVmacEm* replication in primary alveolar macrophages. These data suggest that efficient replication in primary macrophages requires both envelope and long terminal repeat determinants.
我们在此证明，猿猴免疫缺陷病毒（SIVmac）长末端重复序列中的核因子-κB（NF-κB）结合位点对于原代肺泡巨噬细胞中的高效病毒复制至关重要，但对于原代T细胞中的高效复制则是可有可无的。NF-κB位点的突变不会严重损害T细胞嗜性的SIVmac239或巨噬细胞嗜性的SIVmacEm在外周血淋巴细胞或已建立的CD4+细胞系中的复制；然而，NF-κB位点的突变会阻止SIVmacEm在原代肺泡巨噬细胞中的高效复制。这些数据表明，在原代巨噬细胞中的高效复制需要包膜和长末端重复序列决定簇。
