Tsuda M, Karita M, Nakazawa T
Department of Microbiology, Yamaguchi University School of Medicine, Japan.
Microbiol Immunol. 1993;37(1):85-9. doi: 10.1111/j.1348-0421.1993.tb03184.x.
Genetic transformation in Helicobacter pylori was investigated by using its chromosomal and plasmid DNAs. Six out of the eight strains exhibited the natural competence for incorporation of H. pylori chromosomal DNA, and all the strains incorporated the donor DNA efficiently by washing and concentrating the cells with a glycerol solution. The much higher frequency of transformation was obtained in each strain by means of electroporation. Electroporation experiments were also conducted by use of the recombinant DNAs consisting of the H. pylori and Escherichia coli plasmids as the donors, and the occurrence of the homologous recombination was demonstrated between the incoming H. pylori plasmid-derived region and the corresponding region of the originally residing plasmid in H. pylori.
利用幽门螺杆菌的染色体DNA和质粒DNA对其遗传转化进行了研究。8株菌株中有6株表现出摄取幽门螺杆菌染色体DNA的天然感受态，并且通过用甘油溶液洗涤和浓缩细胞，所有菌株都能高效摄取供体DNA。通过电穿孔法，在每株菌株中都获得了更高的转化频率。还使用由幽门螺杆菌和大肠杆菌质粒组成的重组DNA作为供体进行了电穿孔实验，结果表明导入的幽门螺杆菌质粒衍生区域与幽门螺杆菌中原本存在的质粒的相应区域之间发生了同源重组。
