Karlsson J E, Wang S, Rosengren L E, Haglid K G
Institute of Neurobiology, University of Göteborg, Sweden.
Neurochem Res. 1993 Feb;18(2):203-8. doi: 10.1007/BF01474685.
The regional changes in quantities of the glial S-100 protein and the neuron specific enolase in the rat nervous system have been studied after long-term exposure to 2,5-hexanedione. The wet weights of most of the examined nervous tissues were found to be reduced, with an extensive effect seen in the brain stem. Using dot immunobinding assays, the concentrations of S-100 were found to be increased in most of the examined tissues, but unaffected in the brain stem. The total amount of S-100 per tissue was markedly reduced in the brain stem. The content of neuron specific enolase was reduced only in the brain stem. Thus the effects of 2,5-hexanedione on the nervous system varied regionally. The brain stem was severely atrophied with a reduction of neuronal as well as of glial marker proteins. Other brain regions contained increased glial cell marker proteins as signs of progressive astroglial reactions.
长期接触2,5 -己二酮后，对大鼠神经系统中神经胶质S - 100蛋白和神经元特异性烯醇化酶的含量区域变化进行了研究。发现大多数被检查神经组织的湿重减轻，在脑干中观察到广泛影响。使用斑点免疫结合测定法，发现大多数被检查组织中S - 100的浓度升高，但在脑干中未受影响。脑干中每个组织的S - 100总量明显减少。神经元特异性烯醇化酶的含量仅在脑干中降低。因此，2,5 -己二酮对神经系统的影响存在区域差异。脑干严重萎缩，神经元和神经胶质标记蛋白均减少。其他脑区含有增加的神经胶质细胞标记蛋白，作为进行性星形胶质细胞反应的标志。
