构建含有噬菌体λ复制起点的嵌合噬菌体和质粒。
Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.
作者信息
Moore D D, Denniston-Thompson K, Furth M E, Williams B G, Blattner F R
出版信息
Science. 1977 Dec 9;198(4321):1041-6. doi: 10.1126/science.929185.
PMID:929185
Abstract
Segments of the replication control region of bacteriophage lambda (lambda) and lambda mutants defective in replication were attached in vitro to the phi80 phage vector Charon 3 and to the plasmid vector mini Col El (pVH51). The chimeric phages and plasmids have been used to localize the origin of lambda DNA replication and to facilitate a structural analysis of the lambda replicator.
摘要
噬菌体λ（lambda）复制控制区的片段以及复制有缺陷的λ突变体在体外连接到φ80噬菌体载体Charon 3和质粒载体mini Col E1（pVH51）上。这些嵌合噬菌体和质粒已被用于定位λ DNA复制的起点，并有助于对λ复制子进行结构分析。
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