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构建含有噬菌体λ复制起点的嵌合噬菌体和质粒。

Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.

作者信息

Moore D D, Denniston-Thompson K, Furth M E, Williams B G, Blattner F R

出版信息

Science. 1977 Dec 9;198(4321):1041-6. doi: 10.1126/science.929185.

DOI:10.1126/science.929185
PMID:929185
Abstract

Segments of the replication control region of bacteriophage lambda (lambda) and lambda mutants defective in replication were attached in vitro to the phi80 phage vector Charon 3 and to the plasmid vector mini Col El (pVH51). The chimeric phages and plasmids have been used to localize the origin of lambda DNA replication and to facilitate a structural analysis of the lambda replicator.

摘要

噬菌体λ（lambda）复制控制区的片段以及复制有缺陷的λ突变体在体外连接到φ80噬菌体载体Charon 3和质粒载体mini Col E1（pVH51）上。这些嵌合噬菌体和质粒已被用于定位λ DNA复制的起点，并有助于对λ复制子进行结构分析。

相似文献

1
Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.构建含有噬菌体λ复制起点的嵌合噬菌体和质粒。
Science. 1977 Dec 9;198(4321):1041-6. doi: 10.1126/science.929185.
2
Physical structure of the replication origin of bacteriophage lambda.噬菌体λ复制起点的物理结构
Science. 1977 Dec 9;198(4321):1051-6. doi: 10.1126/science.929187.
3
Specificity determinants for bacteriophage lambda DNA replication. II. Structure of O proteins of lambda-phi80 and lambda-82 hybrid phages and of a lambda mutant defective in the origin of replication.
J Mol Biol. 1978 Dec 5;126(2):227-40. doi: 10.1016/0022-2836(78)90360-1.
4
Genetic structure of the replication origin of bacteriophage lambda.
Science. 1977 Dec 9;198(4321):1046-51. doi: 10.1126/science.929186.
5
[Genetic study of bacteriophage phi81. II. Gene localization in the right arm of the phage chromosome and a comparison of phage phi81 with phages lambda and phi80 in regard to gene functions].[噬菌体φ81的遗传学研究。II. 噬菌体染色体右臂上的基因定位以及噬菌体φ81与噬菌体λ和φ80在基因功能方面的比较]
Genetika. 1978;14(7):1146-52.
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Replication of the plasmid pBR322 under the control of a cloned replication origin from the single-stranded DNA phage M13.在来自单链DNA噬菌体M13的克隆复制起点控制下的质粒pBR322的复制。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4638-42. doi: 10.1073/pnas.77.8.4638.
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Lambda phage promoter used to enhance expression of a plasmid-cloned gene.用于增强质粒克隆基因表达的λ噬菌体启动子。
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In vitro construction of bacteriophage lambda and plasmid DNA molecules containing DNA fragments from bacteriophage T4.含有来自噬菌体T4的DNA片段的噬菌体λ和质粒DNA分子的体外构建。
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Construction in vitro of "phage-plasmid" chimerae: a new tool to analyse the mechanism of plasmid maintenance.
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Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.卡戎噬菌体：用于DNA克隆的λ噬菌体更安全的衍生物。
Science. 1977 Apr 8;196(4286):161-9. doi: 10.1126/science.847462.

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二十一种卡隆载体噬菌体的限制酶切图谱。
J Virol. 1980 Jan;33(1):401-10. doi: 10.1128/JVI.33.1.401-410.1980.
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New map of bacteriophage lambda DNA.噬菌体λDNA的新图谱。
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Mol Cell Biol. 1984 Oct;4(10):2072-81. doi: 10.1128/mcb.4.10.2072-2081.1984.
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Genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function.腺相关病毒的遗传分析：体外构建的缺失突变体的特性及腺相关病毒复制功能的证据
J Virol. 1984 Sep;51(3):611-9. doi: 10.1128/JVI.51.3.611-619.1984.
7
Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.用于DNA克隆的杂交噬菌体λCharon载体的构建与特性分析。
J Virol. 1979 Feb;29(2):555-75. doi: 10.1128/JVI.29.2.555-575.1979.
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Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli.转录促进大肠杆菌中由依赖DNA的RNA聚合酶介导的不依赖recA的重组。
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