插入嵌合质粒中的兔α珠蛋白基因的核苷酸序列。
Nucleotide sequences from a rabbit alpha globin gene inserted in a chimeric plasmid.
作者信息
Liu A Y, Paddock G V, Heindell H C, Salser W
出版信息
Science. 1977 Apr 8;196(4286):192-5. doi: 10.1126/science.847466.
PMID:847466
Abstract
Rabbit alpha globin gene copies have been made, using reverse transcriptase and DNA polymerase I, and cloned in bacterial plasmids. Plasmid pHb72 has been shown to contain the alpha gene sequence by restriction enzyme analysis and nucleotide sequencing studies, and therefore has been approved for propagation under P2 plus EK1 conditions by the National REcombinant DNA Committee.
摘要
利用逆转录酶和DNA聚合酶I制备了兔α珠蛋白基因拷贝，并将其克隆到细菌质粒中。通过限制性酶切分析和核苷酸测序研究表明，质粒pHb72含有α基因序列，因此已获国家重组DNA委员会批准在P2加EK1条件下进行增殖。
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