携带兔珠蛋白信使核糖核酸拷贝的质粒的细菌克隆。
Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA.
作者信息
Rabbitts T H
出版信息
Nature. 1976 Mar 18;260(5548):221-5. doi: 10.1038/260221a0.
DOI:10.1038/260221a0
PMID:768776
Abstract
DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.
摘要
兔珠蛋白mRNA的DNA拷贝已被插入到质粒mini-ColE1中。添加聚（dT）尾后，该质粒被用于通过逆转录酶引发mRNA的复制。携带珠蛋白序列的mini-ColE1被变性，并与带有聚（dA）尾的mini-ColE1退火。通过在大肠杆菌中转染分离出含有合成珠蛋白序列的功能性质粒。
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