Labeque R, Mullon C J, Ferreira J P, Lees R S, Langer R
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3476-80. doi: 10.1073/pnas.90.8.3476.
Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modified human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A2. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.
磷脂酶A2（EC 3.1.1.4）可水解低密度脂蛋白（LDL）的某些磷脂。在高胆固醇血症兔中，经磷脂酶A2修饰的人LDL的血浆清除速度比天然人LDL快17倍。使用含有固定化磷脂酶A2的体外循环对高胆固醇血症兔的血脂蛋白进行修饰。经过90分钟的治疗后，观察到血浆胆固醇浓度下降了近30%。治疗后红细胞、白细胞和血小板计数未显示净变化。该技术不需要任何液体置换或吸附剂再生，为降低血清胆固醇和LDL水平提供了一种潜在的方法。
