兔血浆低密度脂蛋白的酶促修饰：一种高胆固醇血症的潜在治疗方法。
Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia.
作者信息
Labeque R, Mullon C J, Ferreira J P, Lees R S, Langer R
机构信息
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3476-80. doi: 10.1073/pnas.90.8.3476.
Abstract
Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modified human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A2. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.
摘要
磷脂酶A2（EC 3.1.1.4）可水解低密度脂蛋白（LDL）的某些磷脂。在高胆固醇血症兔中，经磷脂酶A2修饰的人LDL的血浆清除速度比天然人LDL快17倍。使用含有固定化磷脂酶A2的体外循环对高胆固醇血症兔的血脂蛋白进行修饰。经过90分钟的治疗后，观察到血浆胆固醇浓度下降了近30%。治疗后红细胞、白细胞和血小板计数未显示净变化。该技术不需要任何液体置换或吸附剂再生，为降低血清胆固醇和LDL水平提供了一种潜在的方法。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
3
Efficient trace-labelling of proteins with iodine.用碘对蛋白质进行高效的示踪标记。
Nature. 1958 Jul 5;182(4627):53. doi: 10.1038/182053a0.
4
The theory of tracer experiments with 131I-labelled plasma proteins.用131I标记血浆蛋白的示踪实验理论
Phys Med Biol. 1957 Jul;2(1):36-53. doi: 10.1088/0031-9155/2/1/305.
5
WHHL-rabbit: a low density lipoprotein receptor-deficient animal model for familial hypercholesterolemia.WHHL兔：一种用于家族性高胆固醇血症的低密度脂蛋白受体缺陷动物模型。
FEBS Lett. 1980 Aug 25;118(1):81-4. doi: 10.1016/0014-5793(80)81223-3.
6
Serial inbreeding of rabbits with hereditary hyperlipidemia (WHHL-rabbit).遗传性高脂血症兔（WHHL兔）的连续近亲繁殖。
Atherosclerosis. 1980 Jun;36(2):261-8. doi: 10.1016/0021-9150(80)90234-8.
7
Plasma-exchange therapy of homozygous familial hypercholesterolemia.
N Engl J Med. 1980 Jun 26;302(26):1457-9. doi: 10.1056/NEJM198006263022607.
8
Removal of cholesterol from blood by affinity binding to heparin-agarose: evaluation on treatment in homozygous familial hypercholesterolemia.
Pediatr Res. 1980 Feb;14(2):113-7. doi: 10.1203/00006450-198002000-00009.
9
Hepatic uptake of phospholipid-depleted chylomicrons in vivo. Comparison with the uptake of chylomicron remnants.体内磷脂耗尽的乳糜微粒的肝脏摄取。与乳糜微粒残粒摄取的比较。
Biochem J. 1981 Dec 15;200(3):547-53. doi: 10.1042/bj2000547.
10
An enzymatic system for removing heparin in extracorporeal therapy.
Science. 1982 Jul 16;217(4556):261-3. doi: 10.1126/science.7089564.
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