Ostgaard G, Reed R K
Department of Physiology, University of Bergen, Norway.
Acta Physiol Scand. 1993 Mar;147(3):329-35. doi: 10.1111/j.1748-1716.1993.tb09506.x.
The output of hyaluronan in mesenteric lymph was studied in anaesthetized rats to allow estimation of the turnover rate. The duodenum/jejunum contained 45 micrograms of this glycosaminoglycan per g wet tissue weight. In fasted rats the concentration of hyaluronan in postnodal lymph averaged 15 micrograms ml-1 and the mean efflux was 2.1 micrograms h-1, corresponding to a daily removal of 10% of the intestinal hyaluronan content. An intravenously injected bolus of 0.9% saline 4 ml 100 g-1 followed by an infusion of the same amount per hour increased the hyaluronan concentration in lymph transiently to 22 micrograms ml-1. During the 8-h i.v. infusion the hyaluronan output remained five times above control due to the high lymph flow. Water and hyaluronan content of the small intestine remained unaltered despite the saline load, the maintained tissue level of hyaluronan suggests an increased rate of synthesis.
为了评估透明质酸的周转率，对麻醉大鼠肠系膜淋巴中透明质酸的输出进行了研究。十二指肠/空肠每克湿组织重量含有45微克这种糖胺聚糖。在禁食大鼠中，节后淋巴中透明质酸的浓度平均为15微克/毫升，平均流出量为2.1微克/小时，相当于每天清除肠道透明质酸含量的10%。静脉注射4毫升/100克体重的0.9%生理盐水推注，随后以相同量每小时输注，可使淋巴中透明质酸浓度短暂升高至22微克/毫升。在8小时的静脉输注期间，由于淋巴流量高，透明质酸输出量仍比对照组高五倍。尽管有生理盐水负荷，小肠的水和透明质酸含量仍保持不变，透明质酸组织水平的维持表明合成速率增加。
