Lee J S
Microvasc Res. 1984 May;27(3):370-8. doi: 10.1016/0026-2862(84)90066-9.
A simple procedure for the collection of the postnodal and prenodal hepatic lymph in the rats under pentobarbital anesthesia is described. Lymph flow (JL) from the main hepatic lymph duct ( postnodal ), protein concentration in both the lymph (CL) and plasma (CP), and the CL/CP ratio were 0.22 +/- 0.1 ml/kg of body weight . hr (0.66 +/- 0.3 ml/100 g wet liver wt . hr), 4.3 +/- 0.5 and 6.2 +/- 0.3 g%, and 0.69 +/- 0.1 (mean +/- SE), respectively. There was no significant difference in CL between prenodal and postnodal lymph in the fasted or unfasted rats. During intestinal fluid absorption with an increase in intestinal JL, postnodal hepatic JL or CL did not change. During intravenous saline infusion with lowering of plasma oncotic pressure, there was a small increase in prenodal JL (by approximately 160%) with some decrease (by 16%) in CL/CP ratio but a much larger increase in postnodal JL (by approximately 700%) with a significant decrease (by 55%) in CL/CP ratio. The results demonstrate that both flow and protein concentration of the postnodal lymph differ greatly from that of the prenodal lymph due to modification during transit through the lymph node. The small increase of the prenodal JL with negligible change in CL/CP ratio during saline infusion suggests that oncotic pressure grandient plays a minor or insignificant role in the transsinusoidal fluid transport in the liver.
描述了一种在戊巴比妥麻醉下收集大鼠肝门后和肝门前肝淋巴的简单方法。肝主淋巴管（肝门后）的淋巴流量（JL）、淋巴（CL）和血浆（CP）中的蛋白质浓度以及CL/CP比值分别为0.22±0.1 ml/kg体重·小时（0.66±0.3 ml/100 g湿肝重·小时）、4.3±0.5和6.2±0.3 g%以及0.69±0.1（平均值±标准误）。禁食或未禁食大鼠的肝门前和肝门后淋巴中的CL无显著差异。在肠液吸收期间，随着肠JL增加，肝门后肝JL或CL未发生变化。在静脉输注生理盐水导致血浆胶体渗透压降低期间，肝门前JL有小幅增加（约160%），CL/CP比值有一定降低（16%），但肝门后JL有大幅增加（约700%），CL/CP比值有显著降低（55%）。结果表明，由于在通过淋巴结的过程中发生了改变，肝门后淋巴的流量和蛋白质浓度与肝门前淋巴有很大差异。生理盐水输注期间肝门前JL的小幅增加以及CL/CP比值的变化可忽略不计，这表明胶体渗透压梯度在肝脏跨窦状隙液体转运中起次要或不显著的作用。
