Hugues B, Andre M, Plantat J L, Champsaur H
Laboratoire d'Hygiène et de Biologie de la Ville de Nice, France.
Zentralbl Hyg Umweltmed. 1993 Feb;193(5):440-9.
Enumeration of cultivable virus particle in sewage requires the samples to be concentrated. Two adsorption-elution methods, the glass wool cartridge method and the glass powder fluid layer method were compared. Firstly, it was demonstrated that virus could be readily recovered from the head, first 25 ml, of eluate of glass wool rather than from a reconcentration of the entire eluate, either by organic flocculation: 83% of positivity vs 44% respectively or double precipitation by PEG: 85% of positivity vs 61% respectively. Concentration on glass wool allowed the rescue of a greater number of positive samples (33/35) than on glass powder (16/35) as well as a higher mean titer of indigenous viruses, respectively 19 MPNCU/l vs 6 MPNCU/l (F1(34) = 36.02, P < 0.0001). Enteroviruses were the most frequently isolated viruses: found in 34/35 samples by either method, in 32/35 samples by glass wool, and in 14/35 samples by glass powder. Of the 12 enterovirus types identified by either method, a majority was Echovirus 6 (10 samples), Echovirus 11 (7 samples), Coxsackievirus B5 (4 samples) and also Coxsackievirus A7, A16, A21, B1, B6, Echovirus 1, 4, 12 and 19. Adenovirus types 1 and 5 were isolated from 4 samples by either method.
对污水中可培养病毒颗粒进行计数需要对样本进行浓缩。比较了两种吸附 - 洗脱方法，即玻璃棉柱法和玻璃粉液层法。首先，结果表明，通过有机絮凝从玻璃棉洗脱液的前25毫升中能很容易地回收病毒，而不是从整个洗脱液的再浓缩物中回收，有机絮凝法的阳性率分别为83%和44%，聚乙二醇双沉淀法的阳性率分别为85%和61%。与玻璃粉（16/35）相比，玻璃棉浓缩能挽救更多阳性样本（33/35），并且本地病毒的平均滴度更高，分别为19 MPNCU/l和6 MPNCU/l（F1(34) = 36.02，P < 0.0001）。肠道病毒是最常分离出的病毒：两种方法在34/35的样本中均能检测到，玻璃棉法在32/35的样本中能检测到，玻璃粉法在14/35的样本中能检测到。通过两种方法鉴定出的12种肠道病毒类型中，大多数是艾柯病毒6型（10个样本）、艾柯病毒11型（7个样本）、柯萨奇病毒B5型（4个样本），还有柯萨奇病毒A7型、A16型、A21型、B1型、B6型、艾柯病毒1型、4型、12型和19型。两种方法在4个样本中均分离出1型和5型腺病毒。
