Grinstein S, Melnick J L, Wallis C
Bull World Health Organ. 1970;42(2):291-6.
In order to detect viruses in sewage or streams, it is first necessary to concentrate the virus present in the fluid sample. Available methods are not readily manageable for concentrating virus from large volumes of fluid, and have not always yielded high recovery rates. In the study described in this paper, a method for concentration of viruses by adsorption on insoluble cross-linked maleic anhydride polyelectrolytes has been utilized to survey the viral flora of sewage and of a stream receiving sewage effluents, in a residential area of Houston, Texas. On a single day the virus flow at different points along the stream varied from 304 000 to 6 014 000 PFU/min. From 84 samples each of 1 US gal, 14 520 isolates were obtained, chiefly echovirus type 7 and polioviruses of all 3 types, some of them with characteristics of virulent wild strains. With virus isolation rates as high as those achieved, it is now possible to monitor virus in natural waters more effectively.
为了检测污水或溪流中的病毒，首先需要浓缩液体样本中存在的病毒。现有的方法在从大量液体中浓缩病毒时并不易于操作，而且回收率也并非总是很高。在本文所述的研究中，一种通过吸附在不溶性交联马来酸酐聚电解质上来浓缩病毒的方法，已被用于调查得克萨斯州休斯顿一个居民区的污水和接纳污水排放的溪流中的病毒菌群。在某一天，沿溪流不同地点的病毒流量从304,000到6,014,000空斑形成单位/分钟不等。从84个1美制加仑的样本中，获得了14,520个分离株，主要是7型艾柯病毒和所有3种类型的脊髓灰质炎病毒，其中一些具有强毒野生株的特征。鉴于病毒分离率达到了如此之高的水平，现在有可能更有效地监测天然水体中的病毒。
