Ogrydziak D M
Institute of Marine Resources, University of California, Davis 95616.
Crit Rev Biotechnol. 1993;13(1):1-55. doi: 10.3109/07388559309069197.
Many species of yeast secrete significant amounts of protease(s). In this article, results of numerous surveys of yeast extracellular protease production have been compiled and inconsistencies in the data and limitations of the methodology have been examined. Regulation, purification, characterization, and processing of yeast extracellular proteases are reviewed. Results obtained from the sequences of cloned genes, especially the Saccharomyces cerevisiae Bar protease, the Candida albicans acid protease, and the Yarrowia lipolytica alkaline protease, have been emphasized. Biotechnological applications and the medical relevance of yeast extracellular proteases are covered. Yeast extracellular proteases have potential in beer and wine stabilization, and they probably contribute to pathogenicity of Candida spp. Yeast extracellular protease genes also provide secretion and processing signals for yeast expression systems designed for secretion of heterologous proteins. Coverage of the secretion of foreign proteases such as prochymosin, urokinase, and tissue plasminogen activator by yeast in included.
许多酵母物种会分泌大量的蛋白酶。在本文中，已汇总了众多关于酵母细胞外蛋白酶产生情况的调查结果，并对数据中的不一致性和方法的局限性进行了研究。本文还综述了酵母细胞外蛋白酶的调控、纯化、特性鉴定及加工过程。重点强调了从克隆基因序列中获得的结果，尤其是酿酒酵母的Bar蛋白酶、白色念珠菌的酸性蛋白酶和解脂耶氏酵母的碱性蛋白酶。文中涵盖了酵母细胞外蛋白酶的生物技术应用及其医学相关性。酵母细胞外蛋白酶在啤酒和葡萄酒的稳定性方面具有潜力，并且可能与念珠菌属的致病性有关。酵母细胞外蛋白酶基因还为设计用于分泌异源蛋白的酵母表达系统提供分泌和加工信号。文中还包括了酵母对外源蛋白酶如凝乳酶原、尿激酶和组织型纤溶酶原激活剂的分泌情况。
