Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, 51-630 Wrocław, Poland.
SPO, Univ Montpellier, INRAE, Institut Agro, 34060 Montpellier, France.
Int J Mol Sci. 2023 Mar 30;24(7):6514. doi: 10.3390/ijms24076514.
Proteolytic enzymes are commercially valuable and have multiple applications in various industrial sectors. The most studied proteolytic enzymes produced by , extracellular alkaline protease (Aep) and extracellular acid protease (Axp), were shown to be good candidates for different biotechnological applications. In this study, we performed a comprehensive analysis of the alkaline proteolytic enzymes of clade species, including phylogenetic studies, synteny analysis, and protease production and application. Using a combination of comparative genomics approaches based on sequence similarity, synteny conservation, and phylogeny, we reconstructed the evolutionary scenario of the gene for species of the clade. Furthermore, except for the proteolytic activity of the analyzed clade strains, the brewers' spent grain (BSG) was used as a substrate to obtain protein hydrolysates with antioxidant activity. For each culture, the degree of hydrolysis was calculated. The most efficient protein hydrolysis was observed in the cultures of , , and In contrast, the best results obtained using the 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) method were observed for the culture medium after the growth of , , and on BSG.
蛋白酶在商业上具有很高的价值，在各个工业领域有多种应用。其中研究最多的两种 产生的胞外碱性蛋白酶（Aep）和胞外酸性蛋白酶（Axp），被证明是不同生物技术应用的良好候选者。在这项研究中，我们对 属种的碱性蛋白酶进行了全面分析，包括系统发育研究、基因共线性分析以及蛋白酶的生产和应用。我们使用了基于序列相似性、基因共线性保守性和系统发育的比较基因组学方法的组合，重建了 属种的 基因的进化情景。此外，除了分析的 属种菌株的蛋白酶活性外，我们还使用啤酒糟（BSG）作为底物来获得具有抗氧化活性的蛋白水解产物。对于每种培养物，我们都计算了水解度。在 、 和 的培养物中观察到了最高的蛋白水解效率。相比之下，在 、 和 在 BSG 上生长后的培养基中，使用 2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法观察到了最佳的抗氧化活性结果。
