肺炎克雷伯菌血清型K40产生的荚膜多糖的结构测定。用噬菌体相关内切聚糖酶使多糖解聚后获得的寡糖的核磁共振研究。

Structural determination of the capsular polysaccharide produced by Klebsiella pneumoniae serotype K40. NMR studies of the oligosaccharide obtained upon depolymerisation of the polysaccharide with a bacteriophage-associated endoglycanase.

作者信息

Cescutti P, Toffanin R, Kvam B J, Paoletti S, Dutton G G

机构信息

Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università degli Studi di Trieste, Italy.

出版信息

Eur J Biochem. 1993 Apr 1;213(1):445-53. doi: 10.1111/j.1432-1033.1993.tb17780.x.

DOI:10.1111/j.1432-1033.1993.tb17780.x

PMID:8477716

Abstract

The Klebsiella pneumoniae K40 capsular polysaccharide has been isolated and investigated by use of methylation analysis, specific degradations and NMR spectroscopy. The polysaccharide was depolymerised by a bacteriophage-associated endogalactosidase, and the resulting oligosaccharide was characterised by one-dimensional and two-dimensional NMR spectroscopy and direct chemical ionisation MS. The repeating unit of the K40 capsular polysaccharide was shown to be a linear hexasaccharide with the composition-->3)- alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GlcpA++ +-(1-->2-)- alpha-D-Manp-(1-->2)-alpha-D-Manp-(1-->3)-alpha-D-Galp-(1--> (Rha, rhamnose).

摘要

肺炎克雷伯菌K40荚膜多糖已通过甲基化分析、特异性降解和核磁共振光谱法进行分离和研究。该多糖被一种噬菌体相关的内切半乳糖苷酶解聚，所得寡糖通过一维和二维核磁共振光谱法以及直接化学电离质谱进行表征。结果表明，K40荚膜多糖的重复单元是一种线性六糖，其组成为-->3)-α-L-鼠李糖-(1-->2)-α-L-鼠李糖-(1-->4)-α-D-葡萄糖醛酸++ +-(1-->2-)-α-D-甘露糖-(1-->2)-α-D-甘露糖-(1-->3)-α-D-半乳糖-(1-->（Rha，鼠李糖）。