Sabová L, Zeman I, Supek F, Kolarov J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Eur J Biochem. 1993 Apr 1;213(1):547-53. doi: 10.1111/j.1432-1033.1993.tb17793.x.
The AAC3 gene of Saccharomyces cerevisiae encodes a mitochondrial ADP/ATP translocator which is subject to oxygen repression. Evidence is presented here, that the repression of AAC3 expression is dependent upon heme and the ROX1 factor. The promoter region of the AAC3 gene was isolated, sequenced, and deletion analysis was performed using lacZ as a reporter gene to determine the cis-acting regions responsible for the regulation of AAC3 expression. The results of the deletion analysis show that the negative control of the AAC3 gene by oxygen and ROX1 factor is mediated by an upstream repression sequence consisting of a T-rich segment adjacent to the consensus elements that are present in the 5' flanking regions of several other yeast genes. An additional upstream repressor site was located within the AAC3 promoter which, however, is not related either to oxygen or to ROX1 factor. The data presented here delineate the main cellular factors and DNA sequences involved in the regulatory mechanism by which an essential function for anaerobic cells growth, ADP/ATP translocation, is ensured. In addition, they show that the AAC3 gene belongs to the family of yeast genes including TIF51B, COX5b, HEM13 and CYC7 that are negatively regulated by oxygen and heme.
酿酒酵母的AAC3基因编码一种线粒体ADP/ATP转运体，该转运体受氧抑制。本文提供的证据表明，AAC3表达的抑制取决于血红素和ROX1因子。分离了AAC3基因的启动子区域并进行测序，使用lacZ作为报告基因进行缺失分析，以确定负责调控AAC3表达的顺式作用区域。缺失分析的结果表明，氧和ROX1因子对AAC3基因的负调控是由一个上游抑制序列介导的，该序列由一个富含T的片段组成，该片段与其他几个酵母基因5'侧翼区域中存在的共有元件相邻。在AAC3启动子内还定位了一个额外的上游阻遏位点，然而，该位点与氧或ROX1因子均无关。本文提供的数据描绘了参与调控机制的主要细胞因子和DNA序列，通过该调控机制确保了厌氧细胞生长的基本功能——ADP/ATP转运。此外，它们表明AAC3基因属于酵母基因家族，包括TIF51B、COX5b、HEM13和CYC7，这些基因受氧和血红素的负调控。
