Torii I, Morikawa S, Harada T, Kitamura Y
Department of Pathology First Unit, Shimane Medical University, Izumo, Japan.
Immunology. 1993 Mar;78(3):482-90.
Using mast cell-deficient mutant W/Wv mice and their normal counterpart we re-evaluated the significance of participation of mast cells in allergic inflammatory response. W/Wv mice developed immediate hypersensitivity (IH) footpad reaction (FPR) to a somewhat lesser degree than the normal mice, suggesting that the mast cell might amplify the response. To exert classical tuberculin (tbc) delayed-type hypersensitivity (DTH) mast cells were not an essential cellular component. Vasoactive amines were essential to develop the response, but it did not necessarily originate from mast cells. When mice were immunized with methylated human serum albumin (MHSA) emulsified in incomplete Freund's adjuvant (IFA), mast cells were required to elicit DTH FPR. This was confirmed by the lack of the response in W/Wv mice, and the restoration of FPR by local transplantation of mature mast cells into mutant mice. This mast cell-dependent (MD) DTH was different from tbc DTH as follows: mast cell dependency, macrophage dependency as revealed by ferritin sensitivity, kinetics of sensitization, effect of host's age and histopathology. Thus we concluded that there are two types of DTH in mice; one is macrophage-dependent tbc and the other is mast cell-dependent DTH. The correspondence of the DTH to the Jones-Mote (JM) DTH is discussed, although the dominance of mast cells in MD DTH lesion was not observed.
利用肥大细胞缺陷型突变体W/Wv小鼠及其正常对照，我们重新评估了肥大细胞参与变应性炎症反应的意义。W/Wv小鼠发生速发型超敏反应（IH）足垫反应（FPR）的程度略低于正常小鼠，提示肥大细胞可能增强该反应。对于经典结核菌素（tbc）迟发型超敏反应（DTH），肥大细胞并非必需的细胞成分。血管活性胺对于引发该反应至关重要，但不一定源自肥大细胞。当用不完全弗氏佐剂（IFA）乳化的甲基化人血清白蛋白（MHSA）免疫小鼠时，引发DTH FPR需要肥大细胞。W/Wv小鼠缺乏反应以及将成熟肥大细胞局部移植到突变小鼠中可恢复FPR证实了这一点。这种肥大细胞依赖性（MD）DTH与tbc DTH的不同之处如下：肥大细胞依赖性、铁蛋白敏感性显示的巨噬细胞依赖性、致敏动力学、宿主年龄的影响以及组织病理学。因此我们得出结论，小鼠中有两种类型的DTH；一种是巨噬细胞依赖性tbc，另一种是肥大细胞依赖性DTH。尽管未观察到肥大细胞在MD DTH病变中的优势地位，但讨论了DTH与琼斯 - 莫特（JM）DTH的对应关系。
