使小鼠对化学变应原致敏可调节分离的肥大细胞对IgE依赖性激活的反应性。
Sensitization of mice to chemical allergens modulates the responsiveness of isolated mast cells to IgE-dependent activation.
作者信息
Holliday M R, Dearman R J, Kimber I, Coleman J W
机构信息
Department of Pharmacology and Therapeutics, University of Liverpool, U.K.
出版信息
Immunology. 1993 Mar;78(3):508-10.
It is known that the release of granule-associated inflammatory amines by isolated mouse tissue-type mast cells is subject to regulation in vitro by a number of cytokines that are produced during the immune response. In this study we investigated whether mast cell secretory function might also be subject to regulation in vivo during active sensitization. Mice were sensitized with one of three chemical allergens (trimellitic anhydride, TMA; 2,4-dinitrochlorobenzene, DNCB; or oxazolone) all of which induce contact sensitization and one of which (TMA) in addition causes immediate hypersensitivity. Peritoneal mast cells isolated from treated mice and sensitized passively with IgE released a greater proportion of cellular serotonin (5-HT) on stimulation either by anti-IgE or by specific antigen than did cells isolated from vehicle-treated controls. These results show that the function of mast cells is susceptible in vivo to regulatory influences that result from induction of an immune response.
已知分离出的小鼠组织型肥大细胞释放颗粒相关炎性胺，在体外会受到免疫反应期间产生的多种细胞因子的调节。在本研究中，我们调查了在主动致敏过程中，肥大细胞的分泌功能在体内是否也会受到调节。用三种化学变应原（偏苯三酸酐，TMA；2,4-二硝基氯苯，DNCB；或恶唑酮）之一对小鼠进行致敏，所有这些变应原都会诱发接触性致敏，其中一种（TMA）还会引起速发型超敏反应。从经处理的小鼠中分离出的腹膜肥大细胞，用IgE被动致敏后，在抗IgE或特异性抗原刺激下，比从赋形剂处理的对照小鼠中分离出的细胞释放出更大比例的细胞血清素（5-HT）。这些结果表明，肥大细胞的功能在体内易受免疫反应诱导所产生的调节影响。
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