轴突微管稳定的位点。
Sites of microtubule stabilization for the axon.
作者信息
Baas P W, Ahmad F J, Pienkowski T P, Brown A, Black M M
机构信息
Department of Anatomy, University of Wisconsin Medical School, Madison 53706.
出版信息
J Neurosci. 1993 May;13(5):2177-85. doi: 10.1523/JNEUROSCI.13-05-02177.1993.
We have sought to determine the principal site(s) in the neuron where axonal microtubules (MTs) are stabilized. To accomplish this, we compared the proximal and distal regions of the axon and the axon shaft with regard to their content of newly stabilized MT polymer, using the following criteria. Stable polymer was identified by its resistance to nocodazole, and newly stabilized polymer was distinguished from older stable polymer by the staining of the former but not the latter for tyrosinated alpha-tubulin. Our results indicate that roughly 36.4%, 5.4%, and 2.4% of the total MT mass in the proximal and distal regions of the axon and the axon shaft is newly stabilized, respectively. Thus, while MT stabilization occurs throughout the axon, the proximal region is by far the most active with regard to this process.
我们试图确定神经元中轴突微管（MTs）稳定的主要部位。为实现这一目标，我们使用以下标准，比较了轴突的近端和远端区域以及轴突干中新增稳定MT聚合物的含量。通过对诺考达唑的抗性来鉴定稳定聚合物，通过对前者而非后者的酪氨酸化α-微管蛋白染色，将新增稳定聚合物与较旧的稳定聚合物区分开来。我们的结果表明，轴突近端和远端区域以及轴突干中MT总量的约36.4%、5.4%和2.4%分别是新增稳定的。因此，虽然MT稳定在整个轴突中都会发生，但就这一过程而言，近端区域是最活跃的。
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