Jin B L, Xie L H, Chen D C, Li C L, Xie J T
Department of Biology, Medical Research Center, Nankai University, Tianjin, P.R. China.
Methods Find Exp Clin Pharmacol. 1993 Jan-Feb;15(1):23-9.
The effects of Chinese medicine, alcoholized rhubarb, on cardiac electrophysiology and contractile force were studied by using monophasic action potential (MAP) techniques with an improved pressure-electrical transducer epicardial electrode and/or an endocardial pressure-contact electrode catheter in 27 feline hearts in vivo. The results of the experiments were as follows: 1) Alcoholized rhubarb solution (1.5 g/kg) caused a greater increase in the MAP amplitude (from 18.8 +/- 0.62 mV to 26.7 +/- 3.87 mV, p < 0.01), in the MAP maximum rate of rise of phase 0 (from 1.7 +/- 0.13 V/sec to 2.6 +/- 0.43, p < 0.01), and in the MAP duration at 50% and 90% repolarization (from 194.3 +/- 14.00 msec to 216.3 +/- 6.30 msec and from 238.8 +/- 21.41 msec to 280.5 +/- 14.68 msec, respectively, p < 0.01), and a decrease in the heart rate (from 130.8 +/- 12.10 bpm to 110.8 +/- 10.35 bpm, p < 0.01) in the epicardial monophasic action potentials after rhubarb solution administration for 1 min. 2) The ventricular contractile force of the feline heart in vivo was also significantly increased (11.1 +/- 0.52 g to 14.0 +/- 1.41 g, p < 0.01) by rhubarb solution. 3) A dose-dependent relation of alcoholized rhubarb solution was found and indicated that lower doses (1.0-1.5 g/kg) had an excitatory effect, while a higher dose (2.0 g/kg) had inhibitory or toxic effects on the intact feline heart. The present study provides evidence that Chinese rhubarb solution has electrophysiological effects on the feline heart in vivo.
采用改良的压力 - 电换能器心外膜电极和/或心内膜压力接触电极导管记录单相动作电位（MAP）技术，在27只猫的活体心脏上研究了中药酒大黄对心脏电生理和收缩力的影响。实验结果如下：1）给予酒大黄溶液（1.5 g/kg）1分钟后，心外膜单相动作电位的MAP幅度（从18.8±0.62 mV增至26.7±3.87 mV，p<0.01）、MAP 0期最大上升速率（从1.7±0.13 V/秒增至2.6±0.43，p<0.01）、50%和90%复极化时的MAP持续时间（分别从194.3±14.00毫秒增至216.3±6.30毫秒和从238.8±21.41毫秒增至280.5±14.68毫秒，p<0.01）均显著增加，心率（从130.8±12.10次/分钟降至110.8±10.35次/分钟，p<0.01）显著降低。2）酒大黄溶液还使猫活体心脏的心室收缩力显著增加（从11.1±0.52 g增至14.0±1.41 g，p<0.01）。3）发现酒大黄溶液存在剂量依赖性关系，表明较低剂量（1.0 - 1.5 g/kg）对完整猫心脏有兴奋作用，而较高剂量（2.0 g/kg）有抑制或毒性作用。本研究提供了证据表明大黄溶液对猫活体心脏具有电生理作用。
