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榆树病原菌榆长喙壳菌(广义)的电泳核型

Electrophoretic karyotypes of the elm tree pathogen Ophiostoma ulmi (sensu lato).

作者信息

Dewar K, Bernier L

机构信息

Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Université Laval, Ste-Foy, Québec, Canada.

出版信息

Mol Gen Genet. 1993 Apr;238(1-2):43-8. doi: 10.1007/BF00279529.

Abstract

Pulsed field gel electrophoresis using OFAGE, TAFE, and CHEF systems has been used to more fully characterize karyotypic variation within the two closely related fungal species of Ophiostoma ulmi sensu lato. Twelve wild-type and laboratory strains, representing the less aggressive species O. ulmi and both of the biotypes of the more aggressive species O. novo-ulmi were studied and their karyotypes determined. Depending on the strain, a minimum of four to a minimum of eight chromosomal DNA bands were present that fall into three distinct size classes, with one exception. Strain CESS16K (O. novo-ulmi, North American aggressive subgroup) contains a unique chromosomal DNA band which comigrated near a Saccharomyces cerevisiae chromosome of 0.95 Mb. This unique band was the smallest O. ulmi s. l. chromosomal DNA observed. Seven of the twelve strains shared a common chromosomal DNA banding pattern, whereas each of the other five had a unique karyotype. There was no correlation between chromosome profile and species, as some O. novo-ulmi and O. ulmi strains shared common electrophoretic karyotypes.

摘要

使用OFAGE、TAFE和CHEF系统的脉冲场凝胶电泳已被用于更全面地表征狭义榆长喙壳菌两个密切相关真菌物种内的核型变异。研究了代表侵袭性较弱的榆长喙壳菌物种以及侵袭性较强的新榆长喙壳菌两种生物型的12个野生型和实验室菌株,并确定了它们的核型。根据菌株的不同,存在至少四条至至少八条染色体DNA条带,可分为三个不同的大小类别,但有一个例外。菌株CESS16K(新榆长喙壳菌,北美侵袭性亚组)包含一条独特的染色体DNA条带,该条带与酿酒酵母0.95 Mb的染色体在相近位置共迁移。这条独特的条带是所观察到的狭义榆长喙壳菌最小的染色体DNA。12个菌株中有7个共享一种常见的染色体DNA条带模式,而其他5个菌株中的每一个都有独特的核型。染色体图谱与物种之间没有相关性,因为一些新榆长喙壳菌和榆长喙壳菌菌株共享常见的电泳核型。

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