Bezzubova O, Shinohara A, Mueller R G, Ogawa H, Buerstedde J M
Basel Institute for Immunology, Switzerland.
Nucleic Acids Res. 1993 Apr 11;21(7):1577-80. doi: 10.1093/nar/21.7.1577.
Comparisons of the amino acid sequences of three yeast RecA-like proteins, Rad51 and DMC1 from S.cerevisiae and Rad51 from S.pombe, revealed several highly conserved regions. Degenerated oligonucleotides encoding two of these regions were used for the polymerase chain reaction to clone a chicken RecA-like gene. The encoded protein shares 68% and 49% identical amino acids with the Rad51 and DMC1 proteins. The strong sequence conservation between the yeast and chicken genes indicates that RecA homologues are conserved throughout evolution from prokaryotes to higher eukaryotes. High expression of the chicken Rad51 gene was found within the organs of lymphoid and germ cell development suggesting its involvement in lymphoid and meiotic recombination.
对三种酵母RecA样蛋白（酿酒酵母的Rad51和DMC1以及粟酒裂殖酵母的Rad51）的氨基酸序列进行比较，发现了几个高度保守的区域。编码其中两个区域的简并寡核苷酸用于聚合酶链反应，以克隆鸡的RecA样基因。所编码的蛋白质与Rad51和DMC1蛋白分别有68%和49%的相同氨基酸。酵母和鸡基因之间强烈的序列保守性表明，RecA同源物在从原核生物到高等真核生物的整个进化过程中都是保守的。在淋巴和生殖细胞发育的器官中发现鸡Rad51基因的高表达，表明它参与淋巴和减数分裂重组。
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