Tiboni G M
Clinica Ostetrica e Ginecologica, Università G.D'Annunzio, Chieti, Italy.
Res Commun Chem Pathol Pharmacol. 1993 Mar;79(3):381-4.
The teratogenic potential of the novel bis-triazole antifungal agent fluconazole (F) was investigated on mouse embryos during early organogenesis using the whole-embryo culture system. Early somite stage embryos (4-5 somites) were continuously cultured for 48 hours in rat serum added with 0, 25, 50 or 75 micrograms/ml of F. Doses of 50 and 75 micrograms/ml of F significantly (p < 0.001) impaired morphogenesis, resulting in a 83.3% and 91.6% of embryos showing second branchial arch anomalies, respectively. These findings demonstrate that F induces second branchial arch anomalies in cultured mouse conceptuses. Furthermore, these results may suggest an useful model for the study of arches-derived malformations.
利用全胚胎培养系统，研究了新型双三唑抗真菌剂氟康唑（F）在小鼠胚胎器官形成早期的致畸潜力。将早期体节期胚胎（4 - 5个体节）在添加了0、25、50或75微克/毫升F的大鼠血清中连续培养48小时。50和75微克/毫升的F剂量显著（p < 0.001）损害形态发生，分别导致83.3%和91.6%的胚胎出现第二鳃弓异常。这些发现表明F可诱导培养的小鼠胚胎出现第二鳃弓异常。此外，这些结果可能提示了一个用于研究源自鳃弓畸形的有用模型。
