荧光庆大霉素-碳酸铊琼脂和KF链球菌琼脂用于肉类中肠球菌和粪链球菌计数的比较。
Comparison of fluorescent gentamicin-thallous-carbonate and KF streptococcal agars to enumerate enterococci and fecal streptococci in meats.
作者信息
Knudtson L M, Hartman P A
机构信息
Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
出版信息
Appl Environ Microbiol. 1993 Mar;59(3):936-8. doi: 10.1128/aem.59.3.936-938.1993.
Two selective and differential media were compared for their abilities to enumerate enterococci and fecal streptococci in pork, beef, and poultry products. Counts obtained on KF streptococcal (KF) agar were compared with counts obtained on fluorescent gentamicin-thallous-carbonate (fGTC) agar. Reactions of 13 known enterococcal species were also observed. All 13 species of enterococci as well as Streptococcus bovis and Streptococcus equinus grew equally well on fGTC agar. KF streptococcal medium allowed growth of most species of enterococci but not S. bovis and S. equinus. Quantitative comparisons between the two media inoculated with pure cultures of known species of enterococci revealed equivalent plate counts following incubation. However, when meat samples were plated, counts on fGTC agar were consistently and significantly higher than counts on KF agar for all sample sources.
比较了两种选择性和鉴别性培养基在对猪肉、牛肉和禽肉产品中的肠球菌和粪链球菌进行计数方面的能力。将在KF链球菌（KF）琼脂上获得的计数与在荧光庆大霉素-碳酸铊（fGTC）琼脂上获得的计数进行比较。还观察了13种已知肠球菌的反应。所有13种肠球菌以及牛链球菌和马链球菌在fGTC琼脂上生长同样良好。KF链球菌培养基允许大多数肠球菌生长，但不允许牛链球菌和马链球菌生长。对接种已知肠球菌纯培养物的两种培养基进行的定量比较显示，培养后平板计数相当。然而，当接种肉类样品时，对于所有样品来源，fGTC琼脂上的计数始终显著高于KF琼脂上的计数。
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