Yoshpe-Purer Y
A. Felix Public Health Laboratory, Ministry of Health, Tel-Aviv, Israel.
Appl Environ Microbiol. 1989 Aug;55(8):2041-5. doi: 10.1128/aem.55.8.2041-2045.1989.
The selectivity of KF streptococcus agar (KF) for monitoring fecal streptococci (FS) in seawater was examined in 234 samples of Mediterranean water and compared with the selectivity of M-Enterococcus agar (M-Ent) for 124 samples and with bile-esculin-azide agar (BEA) for 17 samples. KF was found to be unsuitable for marine water because Vibrio alginolyticus and other gram-negative bacilli indigenous to this environment grew well on it and produced red colonies identical to those of FS. In 26% of samples, some with high counts of red colonies on the membrane filters (MF), there were no streptococci, only gram-negative bacilli and staphylococci, and in an additional 23.1% the streptococci constituted less than 50% of the "typical" red colonies on the MF. V. alginolyticus also produced FS-like colonies on MF incubated on BEA but was not isolated from MF incubated on M-Ent. Although staphylococci grew and produced FS-like colonies on all three media, M-Ent was the most selective since no gram-negative bacilli were isolated from MF incubated on it.
对234份地中海水样本检测了用于监测海水中粪链球菌（FS）的KF链球菌琼脂（KF）的选择性，并将其与124份样本的M-肠球菌琼脂（M-Ent）以及17份样本的胆汁七叶苷叠氮琼脂（BEA）的选择性进行了比较。发现KF不适用于海水，因为溶藻弧菌和该环境中的其他革兰氏阴性杆菌在其上生长良好，并产生与FS相同的红色菌落。在26%的样本中，一些在膜滤器（MF）上有大量红色菌落的样本中没有链球菌，只有革兰氏阴性杆菌和葡萄球菌，另外在23.1%的样本中，链球菌在MF上“典型”红色菌落中所占比例不到50%。溶藻弧菌在BEA上孵育的MF上也产生类似FS的菌落，但未从在M-Ent上孵育的MF中分离出来。尽管葡萄球菌在所有三种培养基上均生长并产生类似FS的菌落，但M-Ent的选择性最高，因为在其孵育的MF上未分离出革兰氏阴性杆菌。
