Boden K, Kirchhoff H
Zentralbl Bakteriol Orig A. 1977;237(2-3):342-50.
Proteins extracted with phenol-acetic acid-water (2:1:0.5, w/v/v) from Acholeplasma laidlawii (PG 8), A. granularum (BTS-39), A. oculi (19L), A. modicum (PG 49), A. axanthum (S743) and the Acholeplasma strains C1 and C112 (which were isolated from aborted horse foetuses) were compared by electrophoresis in horizontal acidic polyacylamide flat gel using the electrophoresis equipment LKB Multiphor 2117. In this system the gels are not prepared in the electrophoresis chamber but between glas plates. For electrophoresis they are applied onto a special cooling plate. This makes it possible to produce a number of identical gels (from the same gel mixture and polymerized under the same conditions) what can be important for comparing investigations. The gels can be stored for more than 4 weeks in the refrigerator at +4 degrees C. Marked differences were observed between the electrophoretic patterns of each of the established species and the horse strains C1 and C112. The results are in agreement with those obtained in serological investigations in which the strains C1 and C112 were different from the established Acholeplasma species.
使用LKB Multiphor 2117电泳设备，在水平酸性聚丙烯酰胺平板凝胶中对从莱氏无胆甾原体（PG 8）、颗粒无胆甾原体（BTS - 39）、眼无胆甾原体（19L）、微小无胆甾原体（PG 49）、黄色无胆甾原体（S743）以及从流产马胎儿中分离出的无胆甾原体菌株C1和C112中用苯酚 - 乙酸 - 水（2:1:0.5，w/v/v）提取的蛋白质进行电泳比较。在该系统中，凝胶不是在电泳槽中制备，而是在玻璃板之间制备。进行电泳时，将它们放在一个特殊的冷却板上。这使得能够制备许多相同的凝胶（来自相同的凝胶混合物并在相同条件下聚合），这对于比较研究可能很重要。凝胶可以在4摄氏度的冰箱中保存超过4周。在每种已确定的物种与马菌株C1和C112的电泳图谱之间观察到明显差异。结果与血清学研究中获得的结果一致，在血清学研究中，菌株C1和C112与已确定的无胆甾原体物种不同。
