牛支原体和无胆甾原体参考菌株的电泳特性分析
Electrophoretic characterization of bovine mycoplasma and acholeplasma reference strains.
作者信息
Awaad M H, Krauss H, Schmatz H D
出版信息
Zentralbl Bakteriol Orig A. 1978 Apr;240(3):359-65.
Phenol-acetic acid-water extracts of 13 recognized mycoplasma and acholeplasma reference strains of bovine habitat were subjected to polyacrylamide gel electrophoresis. A characteristic electrophoretic profile was obtained for each strain and the relative mobility (Rm) values of the major protein fractions were determined. All examined strains had a common protein band of 33.3 Rm value. Comparison of electrophoretic patterns obtained from different reference strains revealed 2 to 7 protein bands with the same Rm values in a number of strains. A possible correlation with antigenic relatedness is discussed.
对来自牛栖息地的13种已确认的支原体和无胆甾原体参考菌株的苯酚 - 乙酸 - 水提取物进行聚丙烯酰胺凝胶电泳。获得了每种菌株的特征性电泳图谱，并测定了主要蛋白质组分的相对迁移率（Rm）值。所有检测菌株都有一条Rm值为33.3的共同蛋白带。对不同参考菌株获得的电泳图谱进行比较，发现在许多菌株中有2至7条具有相同Rm值的蛋白带。文中讨论了其与抗原相关性的可能联系。
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