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通过平板凝胶聚丙烯酰胺电泳对禽支原体进行的比较研究(作者译)

[Comparative studies of avian mycoplasmas by flat gel polyacrylamide electrophoresis (author's transl)].

作者信息

Hinz K H, Neumann U

出版信息

Zentralbl Bakteriol Orig A. 1978 Apr;240(2):265-70.

PMID:566006
Abstract

The phenol-acetic-acid extraced cell proteins of Mycoplasma (M.) and Acholeplasma (A.) reference strains (PG31 (M. gallisepticum), PG 16 (M. gallinarum), PG30 (M iners), 17529 (M. meleagridis), WVU 1853 (M. synoviae), 1340 (M. anatis), PG8 and PG9 (A. laidlawii), CKK (Serovar C), DD (Serovar D), WR1 (Serogroup F), 695 (Serogroup I) and 694 (Serogroup L) were anlysed by the flat gel polyacrylamide electrophoresis. With exception of PG8 and PG9 the Coomassie Blue-stained protein patterns show that each of the strains produced reproducible characteristic electrophoretic pattern by which the reference strains could be differentiated. However, before the question could be answered whether the procedure described is suitable to replace the serological species differentiation of avian mycoplasmas, serological and electrophoretic studies of a relevant number of field strain are necessary.

摘要

用平板凝胶聚丙烯酰胺电泳法分析了支原体(M.)和无胆甾原体(A.)参考菌株(PG31(鸡毒支原体)、PG16(鸡支原体)、PG30(矿工支原体)、17529(火鸡支原体)、WVU 1853(滑膜支原体)、1340(鸭支原体)、PG8和PG9(莱氏无胆甾原体)、CKK(血清型C)、DD(血清型D)、WR1(血清群F)、695(血清群I)和694(血清群L))经苯酚-乙酸提取的细胞蛋白。除PG8和PG9外,考马斯亮蓝染色的蛋白质图谱表明,每个菌株都产生了可重复的特征电泳图谱,据此可区分参考菌株。然而,在回答所描述的方法是否适合取代禽支原体血清学种属鉴别这一问题之前,有必要对相当数量的田间菌株进行血清学和电泳研究。

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