Ding J W, Andersson R, Soltesz V, Willén R, Bengmark S
Department of Surgery, Lund University, Sweden.
Eur Surg Res. 1993 Jan-Feb;25(1):11-9. doi: 10.1159/000129252.
Male Sprague-Dawley rats were randomly divided into five groups in which group 1 received a sham operation (controls), groups 2-5 underwent common bile duct ligation and transection 14 days before the experiments. Two days prior to the studies, animals in groups 1 and 2 received saline orally, while groups 3-5 received an oral administration of either cholic acid, deoxycholic acid or whole bile. Specimens were taken for bacterial culture, and blood was collected for endotoxin assay. The rate of positive bacterial cultures from mesenteric lymph nodes in jaundiced saline-treated animals was significantly higher (p < 0.05) as compared with both controls and the other jaundiced animals treated with either bile or bile acids. Assays were positive for endotoxin in the jaundiced saline-treated group, whereas they were negative in both controls and bile- or bile-acid-treated animals. We conclude that oral administration of cholic acid, deoxycholic acid or whole bile inhibited bacterial translocation and endotoxin absorption in obstructive jaundice in the rat.
雄性Sprague-Dawley大鼠被随机分为五组，其中第1组接受假手术（对照组），第2 - 5组在实验前14天进行胆总管结扎和横断。在研究前两天，第1组和第2组动物口服生理盐水，而第3 - 5组口服胆酸、脱氧胆酸或全胆汁。采集标本进行细菌培养，并采集血液进行内毒素检测。与对照组和其他用胆汁或胆汁酸治疗的黄疸动物相比，黄疸生理盐水治疗组动物肠系膜淋巴结细菌培养阳性率显著更高（p < 0.05）。黄疸生理盐水治疗组内毒素检测呈阳性，而对照组以及胆汁或胆汁酸治疗组动物检测呈阴性。我们得出结论，口服胆酸、脱氧胆酸或全胆汁可抑制大鼠梗阻性黄疸中的细菌易位和内毒素吸收。
