在Farr放射免疫分析中通过添加硫酸葡聚糖和氯化钙减少DNA与γ-球蛋白的非特异性结合。
Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride.
作者信息
Wakizaka A, Okuhara E
出版信息
J Immunol Methods. 1979;25(2):119-25. doi: 10.1016/0022-1759(79)90047-4.
PMID:84842
Abstract
The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl2 at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus.
摘要
在碱性pH条件下，添加硫酸葡聚糖或氯化钙可显著降低γ-球蛋白与变性DNA相互作用引起的非特异性结合效应。该方法已被证明适用于检测人类系统性红斑狼疮患者血清中的抗DNA抗体。
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