• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在Farr放射免疫分析中通过添加硫酸葡聚糖和氯化钙减少DNA与γ-球蛋白的非特异性结合。

Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride.

作者信息

Wakizaka A, Okuhara E

出版信息

J Immunol Methods. 1979;25(2):119-25. doi: 10.1016/0022-1759(79)90047-4.

DOI:10.1016/0022-1759(79)90047-4
PMID:84842
Abstract

The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl2 at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus.

摘要

在碱性pH条件下，添加硫酸葡聚糖或氯化钙可显著降低γ-球蛋白与变性DNA相互作用引起的非特异性结合效应。该方法已被证明适用于检测人类系统性红斑狼疮患者血清中的抗DNA抗体。

相似文献

1
Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride.在Farr放射免疫分析中通过添加硫酸葡聚糖和氯化钙减少DNA与γ-球蛋白的非特异性结合。
J Immunol Methods. 1979;25(2):119-25. doi: 10.1016/0022-1759(79)90047-4.
2
Differentiation of specific DNA binding activity of SLE sera from non-specific binding by an addition of dextran sulfate and calcium chloride to the Farr assay system.通过在Farr检测系统中添加硫酸葡聚糖和氯化钙，将系统性红斑狼疮血清的特异性DNA结合活性与非特异性结合区分开来。
Tohoku J Exp Med. 1981 Aug;134(4):357-65. doi: 10.1620/tjem.134.357.
3
The non-specific binding of thermally denatured DNA by healthy human sera in Farr assay with or without addition of dextran sulfate and calcium chloride.在有或没有添加硫酸葡聚糖和氯化钙的情况下，通过法尔检测法检测健康人血清对热变性DNA的非特异性结合。
Tohoku J Exp Med. 1981 Aug;134(4):351-6. doi: 10.1620/tjem.134.351.
4
Clinical significance of anti-nDNA antibodies in ANA-positive systemic lupus erythematosus: comparison of the Farr radioimmunoassay and the Crithidia luciliae immunofluorescent technique.抗核抗体阳性系统性红斑狼疮中抗双链DNA抗体的临床意义：Farr放射免疫分析法与利什曼原虫免疫荧光技术的比较
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1985 Feb;18(1):15-24.
5
Influence of pH on the detection of low- and high-avidity anti-dsDNA.pH对低亲和力和高亲和力抗双链DNA检测的影响。
J Immunol Methods. 1982 Dec 30;55(3):361-73. doi: 10.1016/0022-1759(82)90096-5.
6
A comparison of methods for labelling DNA for use in the radioimmunoassay of DNA-antibodies.用于DNA抗体放射免疫测定的DNA标记方法比较
J Immunol Methods. 1974 May;5(1):1-8. doi: 10.1016/0022-1759(74)90040-4.
7
The Crithidia luciliae and Farr assays for the detection of systemic lupus erythematosus.用于检测系统性红斑狼疮的鲁氏锥虫荧光抗体试验和法尔氏试验。
Ann Clin Lab Sci. 1983 Jan-Feb;13(1):49-54.
8
Microplate ELISA for detection of antibodies to DNA in patients with systemic lupus erythematosus: specificity and correlation with Farr radioimmunoassay.用于检测系统性红斑狼疮患者抗DNA抗体的微孔板酶联免疫吸附测定：特异性及与Farr放射免疫测定的相关性
J Clin Lab Anal. 1989;3(1):34-40. doi: 10.1002/jcla.1860030108.
9
Immunology of DNA. I. The influence of reaction conditions on the Farr assay as used for the detection of anti-ds DNA.DNA的免疫学。I. 反应条件对用于检测抗双链DNA的Farr检测法的影响。
J Immunol Methods. 1976;10(1):27-37. doi: 10.1016/0022-1759(76)90004-1.
10
[Determination of anti dsDNA antibodies by immunofluorescence using Crithidia luciliae. I--Diagnostic and prognostic value in systemic lupus erythematosus. Comparison with the Farr radioimmunoassay].利用鲁氏锥虫通过免疫荧光法测定抗双链DNA抗体。I——在系统性红斑狼疮中的诊断和预后价值。与法尔放射免疫分析法的比较
Rev Rhum Mal Osteoartic. 1984 Apr;51(4):185-91.

引用本文的文献

1
Antigenic structure of DNA: relatively high inhibitory activity of di- and trideoxyribonucleotide derived from DNase digest on the interaction of thermally denatured DNA with systemic lupus erythematosus sera.DNA的抗原结构：脱氧核糖核酸酶消化产生的二脱氧核糖核苷酸和三脱氧核糖核苷酸对热变性DNA与系统性红斑狼疮血清相互作用具有较高的抑制活性。
Clin Exp Immunol. 1981 Feb;43(2):223-30.