Wakizaka A, Kurosaka K, Okuhara E, Kashimura M, Akihama T, Miura A B, Shibata A
Tohoku J Exp Med. 1981 Aug;134(4):351-6. doi: 10.1620/tjem.134.351.
A non-specific DNA binding by human sera was measured in 60 male and female healthy subjects in different ages. Farr assay using thermally denatured mouse embryo [3H]DNA proved a high DNA binding level of 47.9 +/- 5.8% (mean +/- S.D.) on an average of these sera. The addition of dextran sulfate and CaCl2 significantly decreased the mean binding value to 14.7 +/- 4.8% and 6.5 +/- 3.2%, respectively. No significant difference was observed between men and women, or between any age groups from twenties to sixties. The measurement of complement activity in the serum gave no evidence for the participation of complement in the non-specific DNA binding.
在60名不同年龄段的健康男性和女性受试者中检测了人血清的非特异性DNA结合情况。使用热变性小鼠胚胎[3H]DNA的Farr测定法显示，这些血清平均DNA结合水平较高，为47.9±5.8%（平均值±标准差）。添加硫酸葡聚糖和氯化钙后，平均结合值分别显著降低至14.7±4.8%和6.5±3.2%。男性和女性之间，以及二十多岁到六十多岁的任何年龄组之间均未观察到显著差异。血清中补体活性的测定未提供补体参与非特异性DNA结合的证据。
