Kashimura M, Wakizaka A, Kurosaka K, Okuhara E, Akihama T, Miura A B, Shibata A
Tohoku J Exp Med. 1981 Aug;134(4):357-65. doi: 10.1620/tjem.134.357.
A new method for differentiation of specific DNA-binding by human sera from non-specific binding was evaluated with sera from patients with systemic lupus erythematosus in different stages of the disease. An addition of dextran sulfate or calcium chloride to Farr's radioimmunoassay mixture reduced non-specific binding of thermally denatured [3H]DNA of the patient sera without much effect on the specific binding. The measurement of DNA-binding value by the sera in these addition systems provides accurate information with regard to the pathophysiological state of the disease.
采用处于疾病不同阶段的系统性红斑狼疮患者的血清，对一种区分人血清特异性DNA结合与非特异性结合的新方法进行了评估。向法尔放射免疫分析混合物中添加硫酸葡聚糖或氯化钙，可降低患者血清中热变性[3H]DNA的非特异性结合，而对特异性结合影响不大。在这些添加系统中，通过血清测量DNA结合值可提供有关疾病病理生理状态的准确信息。
