Berghen P, Hilderson H, Vercruysse J, Dorny P
Department of Parasitology, Faculty of Veterinary Medicine, Gent, Belgium.
Vet Parasitol. 1993 Feb;46(1-4):175-95. doi: 10.1016/0304-4017(93)90057-t.
Ostertagia ostertagi is widely distributed and is one of the most important parasites affecting young bovine livestock. There is, therefore, a substantial need for sensitive and specific parameters in support of diagnosis of ostertagiasis, especially for subclinical disease related to production losses. In this review, the value and application of pepsinogen, gastrin and antibody response as diagnostic tools are discussed. These three parameters are useful and comparable for confirming clinical disease in calves during their first grazing season. However, their value for detecting subclinical parasitism is questionable. Differences in the course of gastrin and pepsinogen late in the grazing season can be correlated with larval inhibition and the possibility of ostertagiasis Type II. Relatively few serological methods have been developed for the immunodiagnosis of Ostertagia and until now the indirect antibody-detecting enzyme-linked immunosorbent assay (ELISA) has been the method of choice. Antibody measuring methods have several disadvantages, most notably a lack of sensitivity and specificity, which limits their use in longitudinal epidemiological studies. Considering the necessity of cost effectiveness and ease of use, it is anticipated that additional work will result in the enhancement and quality of current immunodiagnostic methods.
奥斯特他吉奥斯特他吉分布广泛，是影响幼年牛类家畜的最重要寄生虫之一。因此，迫切需要灵敏且特异的参数来辅助奥斯特他吉iasis病的诊断，尤其是与生产损失相关的亚临床疾病。在本综述中，讨论了胃蛋白酶原、胃泌素和抗体反应作为诊断工具的价值及应用。这三个参数对于确认犊牛在其第一个放牧季节的临床疾病很有用且具有可比性。然而，它们在检测亚临床寄生虫感染方面的价值存疑。放牧季节后期胃泌素和胃蛋白酶原变化过程中的差异可能与幼虫抑制及II型奥斯特他吉iasis病的可能性相关。针对奥斯特他吉的免疫诊断，相对较少的血清学方法被开发出来，到目前为止，间接抗体检测酶联免疫吸附测定（ELISA）一直是首选方法。抗体检测方法有几个缺点，最明显的是缺乏敏感性和特异性，这限制了它们在纵向流行病学研究中的应用。考虑到成本效益和使用便利性的必要性，预计进一步的工作将提高当前免疫诊断方法的质量。
