Fahy J V, Liu J, Wong H, Boushey H A
Department of Medicine, University of California, San Francisco 94143.
Am Rev Respir Dis. 1993 May;147(5):1126-31. doi: 10.1164/ajrccm/147.5.1126.
To determine the feasibility of cellular and biochemical analysis of sputum induced after inhalation of hypertonic (3%) saline, we analyzed sputum induced in 10 healthy and in 18 asthmatic subjects. We also analyzed saliva samples from all subjects. The entire sputum sample and the saliva sample were reduced using dithiothreitol, and cell counts and differentials were determined. Biochemical analysis was performed on sputum and saliva supernatants obtained after centrifugation. We found that induced sputum from asthmatic subjects had a higher percentage of eosinophils [8.1 +/- 3.43 (mean +/- SEM) versus 0.03 +/- 0.02%, p < 0.009] (after excluding squamous cells) and also had higher levels of albumin (232.3 +/- 54.8 versus 79.5 +/- 9.7 micrograms/ml, p < 0.02), fibrinogen (44.2 +/- 11.6 versus 11.9 +/- 2.5 micrograms/ml, p < 0.008) and eosinophil cationic protein (ECP) (142.6 +/- 34.2 versus 26.1 +/- 4.7 ng/ml, p < 0.006) but not of histamine or tryptase. In saliva, squamous cells made up more than 99% of the cells in both groups, and protein concentrations were not significantly different. We conclude that cellular and biochemical analysis of induced sputum is feasible in healthy and in asthmatic subjects and that it reveals differences similar to those reported from analyses of bronchial lavage fluid.
为了确定吸入高渗（3%）盐水后诱导痰液进行细胞和生化分析的可行性，我们对10名健康受试者和18名哮喘受试者诱导的痰液进行了分析。我们还分析了所有受试者的唾液样本。使用二硫苏糖醇对整个痰液样本和唾液样本进行还原，然后进行细胞计数和分类。对离心后获得的痰液和唾液上清液进行生化分析。我们发现，哮喘受试者诱导痰液中的嗜酸性粒细胞百分比更高[8.1±3.43（平均值±标准误）对0.03±0.02%，p<0.009]（排除鳞状细胞后），白蛋白水平也更高（232.3±54.8对79.5±9.7微克/毫升，p<0.02）、纤维蛋白原（44.2±11.6对11.9±2.5微克/毫升，p<0.008）和嗜酸性粒细胞阳离子蛋白（ECP）（142.6±34.2对26.1±4.7纳克/毫升，p<0.006），但组胺或类胰蛋白酶水平无差异。在唾液中，两组鳞状细胞均占细胞总数的99%以上，蛋白质浓度无显著差异。我们得出结论，在健康受试者和哮喘受试者中，诱导痰液的细胞和生化分析是可行的，并且揭示了与支气管灌洗流体分析报告的差异相似的差异。
