Fermand J P, Mitjavila M T, Le Couedic J P, Tsapis A, Berger R, Modigliani R, Seligmann M, Brouet J C, Vainchenker W
Service d'Immuno-Hématologie, Hôpital Saint Louis, Paris, France.
Br J Haematol. 1993 Mar;83(3):359-64. doi: 10.1111/j.1365-2141.1993.tb04657.x.
We studied two patients with a leukaemic T cell lymphoma who presented with a marked increase in blood eosinophilia. To investigate the mechanism of the eosinophilia, supernatants of peripheral blood cells containing more than 80% lymphoma cells were tested by biological assays for the presence of colony stimulating factors (CSF). In one case supernatants stimulated the growth of granulocyte-macrophage (GM), erythroid and eosinophil colonies. These effects were neutralized by anti-GM-CSF antibodies; anti-IL5 antibodies slightly decreased eosinophil colony formation. Supernatants derived from the second patient cells stimulated the same lineages. Neutralizing experiments demonstrated that in addition to GM-CSF it contained interleukin 3 (IL-3) and interleukin 5 (IL-5). In agreement with the biological data, RNA studies using the polymerase chain reaction showed that cells from the first patient expressed GM-CSF transcripts; IL-5 transcripts were also detected in very low amounts. GM-CSF, IL-3 and IL-5 transcripts were detected in cells from the second patient. Thus eosinophilia associated with some T cell lymphoma is likely due to secretion of different combinations of cytokines by malignant cells.
我们研究了两名患有白血病性T细胞淋巴瘤且血液嗜酸性粒细胞显著增多的患者。为了探究嗜酸性粒细胞增多的机制，我们通过生物学检测对含有超过80%淋巴瘤细胞的外周血细胞上清液进行了集落刺激因子（CSF）检测。在一个病例中，上清液刺激了粒细胞 - 巨噬细胞（GM）、红系和嗜酸性粒细胞集落的生长。这些作用被抗GM - CSF抗体中和；抗IL5抗体略微降低了嗜酸性粒细胞集落的形成。来自第二名患者细胞的上清液刺激了相同的细胞系。中和实验表明，除了GM - CSF外，它还含有白细胞介素3（IL - 3）和白细胞介素5（IL - 5）。与生物学数据一致，使用聚合酶链反应的RNA研究表明，第一名患者的细胞表达GM - CSF转录本；也检测到极少量的IL - 5转录本。在第二名患者的细胞中检测到了GM - CSF、IL - 3和IL - 5转录本。因此，与某些T细胞淋巴瘤相关的嗜酸性粒细胞增多可能是由于恶性细胞分泌不同组合的细胞因子所致。
