[正常条件及γ射线照射后大鼠胸腺细胞染色质与核中性脂质的代谢]
[Metabolism of nuclear neutral lipids and rat thymocyte chromatin under normal conditions and after gamma-irradiation].
作者信息
Kulagina T P, Shuruta S A, Kolomiĭtseva I K
出版信息
Biokhimiia. 1993 Feb;58(2):295-300.
The levels and specific radioactivities of cholesterol and free fatty acids in nuclei and chromatin of thymocytes from normal and gamma-irradiated (10 Gy) rats have been studied. The radioactivity of the total lipid fraction of gamma-irradiated cells was decreased significantly in the absence of inhibition of [2-14C]acetate incorporation into the total protein and lipid reactions and the [3H]uracil incorporation into the acid-insoluble RNA. The concentration of free fatty acids in the nuclei increased significantly after irradiation. The specific radioactivity of cholesterol in chromatin was higher than in the nuclei. The differences in specific radioactivities of free fatty acids were less pronounced. After irradiation the ratio of specific radioactivities of free fatty acids in chromatin to that in the nuclei showed a tendency to increase.
对正常和γ射线照射（10 Gy）大鼠胸腺细胞核及染色质中胆固醇和游离脂肪酸的含量及比放射性进行了研究。在不抑制[2-¹⁴C]乙酸掺入总蛋白和脂质反应以及[³H]尿嘧啶掺入酸不溶性RNA的情况下，γ射线照射细胞的总脂质部分的放射性显著降低。照射后细胞核中游离脂肪酸的浓度显著增加。染色质中胆固醇的比放射性高于细胞核中的比放射性。游离脂肪酸比放射性的差异不太明显。照射后，染色质中游离脂肪酸与细胞核中游离脂肪酸的比放射性之比呈增加趋势。
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