Kulagina T P
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Biochemistry (Mosc). 1997 Sep;62(9):1034-8.
Contents and radioactivity (as incorporation of [2-14C]acetate) of thymocyte nuclear and chromatin lipids were investigated in rats subjected to chronic gamma-irradiation in a dose 10 Gy at the intensity of 12.9 cGy/day. The incorporation of label into total fraction of lipids of nuclei and chromatin and into total phospholipid fraction of thymocyte nuclei increased. However, in the whole cells differences in radioactivity of these fractions were not found. The amount of fatty acids in thymocytes from irradiated rats was decreased but synthesis of fatty acids was activated. The amount of fatty acids in nuclei and chromatin remained unchanged compared with control, whereas radioactivity was significantly higher than in the whole cells. In irradiated thymocytes the amounts of phosphatidylserine in nuclei and phosphatidylserine and phosphatidylinositol in chromatin were increased. The incorporation of [2-14C]acetate into proteins increased in thymocytes of irradiated rats. The role of lipid metabolism in genome functioning is discussed.
对每天以12.9 cGy的剂量接受10 Gy慢性γ射线照射的大鼠胸腺细胞核和染色质脂质的含量及放射性（以[2-14C]乙酸盐的掺入量表示）进行了研究。标记物掺入细胞核和染色质脂质的总组分以及胸腺细胞核总磷脂组分中的量增加。然而，在整个细胞中未发现这些组分放射性的差异。照射大鼠胸腺细胞中的脂肪酸含量降低，但脂肪酸合成被激活。与对照组相比，细胞核和染色质中的脂肪酸含量保持不变，而放射性显著高于整个细胞。在受照射的胸腺细胞中，细胞核中的磷脂酰丝氨酸以及染色质中的磷脂酰丝氨酸和磷脂酰肌醇的含量增加。照射大鼠胸腺细胞中[2-14C]乙酸盐掺入蛋白质中的量增加。讨论了脂质代谢在基因组功能中的作用。
