Faure P, Corticelli P, Richard M J, Arnaud J, Coudray C, Halimi S, Favier A, Roussel A M
Laboratoire de Biochimie C, Centre hospitalier régional universitaire A, Grenoble, France.
Clin Chem. 1993 May;39(5):789-93.
Lipid peroxidation is known to accelerate aging and microvascular lesions in diabetic patients. We studied the acute influence of improved glycemic control on the concentrations of plasma lipid peroxidation intermediates [malondialdehydes (MDA), organic hydroperoxides (OHP)] in ketotic insulin-dependent diabetic patients, as well as the interplay of enzymes such as glutathione peroxidase (GPX) and CuZn superoxide dismutase (CuZn-SOD), and trace elements (Zn, Se, Cu) postulated to be involved in free radical protection. These plasma components were measured on the first day of hospitalization (T0) and when the patient had attained a stable glycemic state after continuous insulin treatment (T1). Plasma MDA and OHP concentrations were high at the beginning of the study but approached reference values after glycemic equilibration. Plasma zinc concentrations were significantly (P < 0.05) decreased during the ketotic state, but also approached reference values with glycemic equilibration. Plasma selenium concentrations and GPX activity were relatively unchanged between T0 and T1. Erythrocyte GPX activity measured at T1 in six patients was below the reference values, whereas CuZn-SOD activity was not affected. Our results show that enhanced lipid peroxidation is associated with decreased zinc plasma concentrations in ketotic patients and underline the beneficial effects of continuous insulin infusion. The mechanisms involved are still unclear but may indicate the importance of extracellular zinc transfer secondary to glycemic disorders.
已知脂质过氧化会加速糖尿病患者的衰老和微血管病变。我们研究了血糖控制改善对酮症胰岛素依赖型糖尿病患者血浆脂质过氧化中间产物[丙二醛（MDA）、有机氢过氧化物（OHP）]浓度的急性影响，以及谷胱甘肽过氧化物酶（GPX）和铜锌超氧化物歧化酶（CuZn-SOD）等酶以及假定参与自由基保护的微量元素（锌、硒、铜）之间的相互作用。在住院第一天（T0）以及患者在持续胰岛素治疗后达到稳定血糖状态时（T1）测量这些血浆成分。研究开始时血浆MDA和OHP浓度较高，但血糖平衡后接近参考值。酮症状态期间血浆锌浓度显著（P<0.05）降低，但血糖平衡后也接近参考值。T0和T1之间血浆硒浓度和GPX活性相对未变。在T1时测量的6名患者的红细胞GPX活性低于参考值，而CuZn-SOD活性未受影响。我们的结果表明，脂质过氧化增强与酮症患者血浆锌浓度降低有关，并强调了持续胰岛素输注的有益作用。其中涉及的机制仍不清楚，但可能表明血糖紊乱继发的细胞外锌转运的重要性。
