Lenz A G, Meyer B, Costabel U, Maier K
Projekt Inhalation, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.
Electrophoresis. 1993 Mar;14(3):242-4. doi: 10.1002/elps.1150140141.
Proteins of human bronchoalveolar lavage fluids, obtained by washing the epithelial lining fluid of the lungs with phosphate-buffered saline, were analyzed by two-dimensional electrophoresis under denaturating and reducing conditions. The two-dimensional pattern of bronchoalveolar lavage fluid proteins of healthy volunteers (controls) were compared with those of patients with idiopathic pulmonary fibrosis, sarcoidosis, and asbestosis. Particular interest was paid to the proteins present in minor amounts mainly in the low molecular weight region of the gels. Marked changes in single protein spots were observed. In idiopathic pulmonary fibrosis the spot intensity of the surfactant-associated protein, SP-A, showing isomeric forms both in charge and in molecular weight, was markedly decreased. In sarcoidosis, the immunoglobulins (IgG, IgA) and a group of protein spots at an isoelectric point of 4.5-5.0 and a molecular mass of 55 kDa were increased. An additional spot appeared at an isoelectric point of 4.5 and a molecular mass of 12 kDa. In particular in asbestosis, but also in some cases of idiopathic pulmonary fibrosis and sarcoidosis, the number and intensity of low molecular weight proteins were increased strongly.
通过用磷酸盐缓冲盐水冲洗肺上皮衬液获得的人支气管肺泡灌洗液体中的蛋白质，在变性和还原条件下通过二维电泳进行分析。将健康志愿者（对照组）支气管肺泡灌洗液体蛋白质的二维图谱与特发性肺纤维化、结节病和石棉沉着病患者的图谱进行比较。特别关注主要存在于凝胶低分子量区域的少量蛋白质。观察到单个蛋白质斑点有明显变化。在特发性肺纤维化中，表面活性剂相关蛋白SP-A的斑点强度明显降低，SP-A在电荷和分子量上均显示异构体形式。在结节病中，免疫球蛋白（IgG、IgA）以及一组等电点为4.5-5.0、分子量为55 kDa的蛋白质斑点增加。在等电点为4.5、分子量为12 kDa处出现一个额外的斑点。特别是在石棉沉着病中，以及在一些特发性肺纤维化和结节病病例中，低分子量蛋白质的数量和强度显著增加。
