Hunt C R, Gasser D L, Chaplin D D, Pierce J C, Kozak C A
Washington University School of Medicine, St. Louis, Missouri 63108.
Genomics. 1993 Apr;16(1):193-8. doi: 10.1006/geno.1993.1158.
The 70,000-D heat shock protein (HSP70) gene family includes both heat-inducible and constitutively expressed genes. We have mapped five murine HSP70 genes to specific sites on three separate chromosomes. Southern blot analysis of Chinese hamster x mouse somatic cell DNAs was used to assign the gene for the 78,000-D glucose-regulated protein (Grp78) to Chromosome (Chr) 2, the male germ cell-specific Hsp70-2 and Hsc70t genes to Chr 12 and Chr 17, respectively, and the heat-inducible Hsp70-3 gene also to Chr 17. Southern blot analysis of DNA from the progeny of two multilocus crosses confirmed the Grp78 location on Chr 2 and suggested the order: centromere-Vim-Abl-Grp78-Hc. Similar analysis also confirmed the initial Hsp70-2 assignment to Chr 12 with the order: Hsp70-2-Aat-Igh. The Hsp70-3 and Hsc70t genes on Chr 17, along with the heat-inducible Hsp70-1 gene, were further localized by Southern blot analysis of genomic clones to the H-2 histocompatibility region with the order: Hsp70-1-Hsp70-3-Hsc70t-Bat-6 (human G7a, valyl-tRNA synthetase).
70000 道尔顿热休克蛋白（HSP70）基因家族包括热诱导型基因和组成型表达基因。我们已将五个小鼠 HSP70 基因定位到三条不同染色体上的特定位点。利用中国仓鼠与小鼠体细胞 DNA 的 Southern 印迹分析，将 78000 道尔顿葡萄糖调节蛋白（Grp78）的基因定位到第 2 号染色体，雄性生殖细胞特异性 Hsp70 - 2 和 Hsc70t 基因分别定位到第 12 号和第 17 号染色体，热诱导型 Hsp70 - 3 基因也定位到第 17 号染色体。对两个多位点杂交后代的 DNA 进行 Southern 印迹分析，证实了 Grp78 位于第 2 号染色体上，并提示其顺序为：着丝粒 - Vim - Abl - Grp78 - Hc。类似分析也证实了最初将 Hsp70 - 2 定位到第 12 号染色体，顺序为：Hsp70 - 2 - Aat - Igh。通过对基因组克隆进行 Southern 印迹分析，进一步将第 17 号染色体上的 Hsp70 - 3 和 Hsc70t 基因，以及热诱导型 Hsp70 - 1 基因定位到 H - 2 组织相容性区域，顺序为：Hsp70 - 1 - Hsp70 - 3 - Hsc70t - Bat - 6（人类 G7a，缬氨酰 - tRNA 合成酶）。
